大肠杆菌分解代谢物基因激活蛋白（CAP）的共有DNA位点：CAP对共有DNA位点的亲和力比对大肠杆菌乳糖操纵子DNA位点的亲和力高450倍。
Consensus DNA site for the Escherichia coli catabolite gene activator protein (CAP): CAP exhibits a 450-fold higher affinity for the consensus DNA site than for the E. coli lac DNA site.
作者信息
Ebright R H, Ebright Y W, Gunasekera A
机构信息
Department of Chemistry, Rutgers University, New Brunswick, NJ 08855.
出版信息
Nucleic Acids Res. 1989 Dec 25;17(24):10295-305. doi: 10.1093/nar/17.24.10295.
Abstract
We have synthesized two 40 base pair DNA fragments; one fragment contains the consensus DNA site for CAP (fragment 'ICAP'); the other fragment contains the E. coli lac promoter DNA site for CAP (fragment 'LCAP'). We have investigated the binding of CAP to the two DNA fragments using the nitrocellulose filter binding assay. Under standard conditions [( NaCl] = 200 mM, pH = 7.3), CAP exhibits a 450-fold higher affinity for ICAP than for LCAP. The salt dependence of the binding equilibrium indicates that CAP makes eight ion pairs with ICAP, but only six ion pairs with LCAP. Approximately half of the difference in binding free energy for interaction of CAP with ICAP vs. LCAP is attributable to this difference in ion-pair formation. The pH dependence of the binding equilibrium indicates that the eight CAP-ICAP ion pairs and the six CAP-LCAP ion pairs do not involve His residues of CAP.
摘要
我们合成了两个40个碱基对的DNA片段；一个片段包含CAP的共有DNA位点（片段“ICAP”）；另一个片段包含大肠杆菌乳糖启动子的CAP DNA位点（片段“LCAP”）。我们使用硝酸纤维素滤膜结合试验研究了CAP与这两个DNA片段的结合。在标准条件下（[NaCl]=200 mM，pH = 7.3），CAP对ICAP的亲和力比对LCAP高450倍。结合平衡的盐依赖性表明，CAP与ICAP形成八个离子对，但与LCAP仅形成六个离子对。CAP与ICAP相互作用和与LCAP相互作用的结合自由能差异中，约一半可归因于离子对形成的这种差异。结合平衡的pH依赖性表明，八个CAP-ICAP离子对和六个CAP-LCAP离子对不涉及CAP的组氨酸残基。
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Consensus DNA site for the Escherichia coli catabolite gene activator protein (CAP): CAP exhibits a 450-fold higher affinity for the consensus DNA site than for the E. coli lac DNA site.大肠杆菌分解代谢物基因激活蛋白（CAP）的共有DNA位点：CAP对共有DNA位点的亲和力比对大肠杆菌乳糖操纵子DNA位点的亲和力高450倍。
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本文引用的文献
1
Proton nuclear magnetic resonance study of the histidine residues of the Escherichia coli adenosine cyclic 3',5'-phosphate receptor protein. pH titration behavior, deuterium exchange, and partial assignments.
Biochemistry. 1982 Aug 17;21(17):4048-53. doi: 10.1021/bi00260a021.
2
The change of DNA structure by specific binding of the cAMP receptor protein from rotation diffusion and dichroism measurements.通过旋转扩散和二色性测量，由环磷酸腺苷受体蛋白的特异性结合引起的DNA结构变化。
EMBO J. 1984 Dec 1;3(12):2873-8. doi: 10.1002/j.1460-2075.1984.tb02223.x.
3
Molecular basis of DNA sequence recognition by the catabolite gene activator protein: detailed inferences from three mutations that alter DNA sequence specificity.分解代谢物基因激活蛋白识别DNA序列的分子基础：来自三个改变DNA序列特异性的突变的详细推断
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7274-8. doi: 10.1073/pnas.81.23.7274.
4
A model for the non-specific binding of catabolite gene activator protein to DNA.一种分解代谢基因激活蛋白与DNA非特异性结合的模型。
Nucleic Acids Res. 1984 Nov 26;12(22):8475-87. doi: 10.1093/nar/12.22.8475.
5
Model of specific complex between catabolite gene activator protein and B-DNA suggested by electrostatic complementarity.由静电互补性提出的分解代谢物基因激活蛋白与B-DNA之间特定复合物的模型。
Proc Natl Acad Sci U S A. 1984 Jul;81(13):3973-7. doi: 10.1073/pnas.81.13.3973.
6
Cyclic AMP receptor protein: role in transcription activation.环磷酸腺苷受体蛋白：在转录激活中的作用。
Science. 1984 May 25;224(4651):831-8. doi: 10.1126/science.6372090.
7
Equilibrium studies of the cyclic AMP receptor protein-DNA interaction.环磷酸腺苷受体蛋白与DNA相互作用的平衡研究。
J Mol Biol. 1984 Jan 25;172(3):241-62. doi: 10.1016/s0022-2836(84)80025-x.
8
On the different binding affinities of CRP at the lac, gal and malT promoter regions.关于CRP在乳糖、半乳糖和麦芽糖操纵子启动子区域的不同结合亲和力。
Nucleic Acids Res. 1983 Nov 25;11(22):7833-52. doi: 10.1093/nar/11.22.7833.
9
Thermodynamic parameters governing interaction of EcoRI endonuclease with specific and nonspecific DNA sequences.控制EcoRI核酸内切酶与特异性和非特异性DNA序列相互作用的热力学参数。
J Biol Chem. 1983 Aug 25;258(16):9820-5.
10
DNA-binding proteins.DNA结合蛋白
Science. 1983 Sep 9;221(4615):1020-6. doi: 10.1126/science.6308768.
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