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利用稳定同位素技术对身份不明尸体进行溯源——案例研究展示

Provenancing of unidentified corpses by stable isotope techniques - presentation of case studies.

作者信息

Lehn Christine, Rossmann Andreas, Graw Matthias

机构信息

Dept. of Forensic Medicine, Ludwig-Maximilians University, Munich, Germany.

Isolab GmbH, Laboratory for Stable Isotope Analyses, Schweitenkirchen, Germany.

出版信息

Sci Justice. 2015 Jan;55(1):72-88. doi: 10.1016/j.scijus.2014.10.006. Epub 2014 Nov 7.

Abstract

Stable isotope methods can be used to determine the provenance of unidentified corpses. Body tissue materials such as teeth, bone, hair and nail taken from mortal remains provide information of different time periods of an individuals' life from childhood to death. Tissues of newborns contain provenance information of different time periods during pregnancy of the child's mother. The results of stable isotope analyses of body residues of two adults and a newborn found in Germany between 2010 and 2012 are presented. To determine the geographic origin and movements of unknown individuals, stable isotopes of hydrogen, carbon, nitrogen and sulphur were analysed in hair and bone collagen samples. Amino acid composition and, as a consequence, δ2H, δ13C, δ15N, and δ34S values in human keratin and bone collagen are different. Consequently correction factors were determined to compare isotopic data of bone collagen with those of an extensive worldwide reference hair collection. The isotopic signatures in hair and in bone collagen samples were compared to geographical groups of reference hair samples by canonical discriminant analysis. The results served as the basis for providing provenance constraints for the unidentified persons as requested by the police and prosecution. Ultimately the individuals were identified; hence the isotopic provenance interpretations can be critically evaluated and are shown to be successful.

摘要

稳定同位素方法可用于确定身份不明尸体的来源。从遗体上获取的牙齿、骨骼、毛发和指甲等身体组织材料能提供个体从童年到死亡不同时期的信息。新生儿的组织包含其母亲孕期不同时间段的来源信息。本文展示了2010年至2012年间在德国发现的两名成年人和一名新生儿遗体的稳定同位素分析结果。为确定身份不明个体的地理来源和活动情况,对毛发和骨胶原样本中的氢、碳、氮和硫的稳定同位素进行了分析。人类角蛋白和骨胶原中的氨基酸组成以及相应的δ2H、δ13C、δ15N和δ34S值各不相同。因此确定了校正因子,以便将骨胶原的同位素数据与广泛的全球参考毛发样本的数据进行比较。通过典型判别分析,将毛发和骨胶原样本中的同位素特征与参考毛发样本的地理分组进行了比较。这些结果为应警方和检方要求为身份不明人员提供来源限制提供了依据。最终这些个体被确认身份;因此同位素来源解释可以得到严格评估,并证明是成功的。

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