Song Xu, Zhang Yuetian, Yin Zhongqiong, Zhao Xinghong, Liang Xiaoxia, He Changliang, Yin Lizi, Lv Cheng, Zhao Ling, Ye Gang, Shi Fei, Shu Gang, Jia Renyong
(a)Natural Medecine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya׳an, 625014, China.
(a)Natural Medecine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya׳an, 625014, China.
Virology. 2015 Feb;476:316-322. doi: 10.1016/j.virol.2014.12.030. Epub 2015 Jan 9.
Newcastle disease virus (NDV) belonging to the Paramyxovirinae subfamily is one of the most devastating pathogens in poultry. Although vaccines are widely applied to control the infection, outbreaks of Newcastle disease (ND) repeatedly happen. Currently, there are no alternative control measures available for ND. In the present study, we found that sulfated Chuanmingshen violaceum polysaccharide (sCVPS) were potent inhibitors of NDV in specific pathogen free chickens infected with a virulent strain. With sCVPS treatment, the survival rate increased by almost 20% and virus titers in test organs, including brain, lung, spleen and thymus, were significantly decreased. The sCVPS also exhibited the ability to prevent viral transmission by reducing the amount of virus shed in saliva and feces. Higher concentrations of interferon α and γ in serum were detected in chickens treated with sCVPS, indicating that one of the antiviral mechanisms may be attributed to the property of immunoenhancement. Histopathological examination showed that sCVPS could alleviate the tissue lesions caused by NDV infection. These results suggest that sCVPS are expected to be a new alternative control measure for NDV infection and further studies could be carried out to evaluate the antiviral activity of sCVPS against other paramyxoviruses.
新城疫病毒（NDV）属于副粘病毒亚科，是家禽中最具毁灭性的病原体之一。尽管疫苗被广泛应用于控制感染，但新城疫（ND）疫情仍反复发生。目前，尚无针对ND的替代控制措施。在本研究中，我们发现硫酸化川明参紫堇多糖（sCVPS）是感染强毒株的无特定病原体鸡中NDV的有效抑制剂。经sCVPS处理后，存活率提高了近20%，包括脑、肺、脾和胸腺在内的受试器官中的病毒滴度显著降低。sCVPS还表现出通过减少唾液和粪便中排出的病毒量来防止病毒传播的能力。在用sCVPS处理的鸡中检测到血清中较高浓度的干扰素α和γ，表明抗病毒机制之一可能归因于免疫增强特性。组织病理学检查表明，sCVPS可以减轻NDV感染引起的组织损伤。这些结果表明，sCVPS有望成为NDV感染的一种新的替代控制措施，并且可以进一步开展研究以评估sCVPS对其他副粘病毒的抗病毒活性。
