Wilczek Piotr, Lesiak Anna, Niemiec-Cyganek Aleksandra, Kubin Barbara, Slomski Ryszard, Nozynski Jerzy, Wilczek Grazyna, Mzyk Aldona, Gramatyka Michalina
Bioengineering Laboratory, Heart Prosthesis Institute, Wolnosci 345a, 41-800, Zabrze, Poland,
J Mater Sci Mater Med. 2015 Jan;26(1):5329. doi: 10.1007/s10856-014-5329-7. Epub 2015 Jan 11.
The aim of the study was to estimate the biomechanical properties of heart valves conduit derived from transgenic pigs to determine the usefulness for the preparation of tissue-engineered heart valves. The acellular aortic and pulmonary valve conduits from transgenic pigs were used to estimate the biomechanical properties of the valve. Non-transgenic porcine heart valve conduits were used as a reference. The biomechanics stability of acellular valve conduits decreased both for the transgenic and non-transgenic porcine valves. The energy required to break the native pulmonary valve derived from transgenic pigs was higher (20,475 ± 7,600 J m(-2)) compared with native non-transgenic pigs (12,140 ± 5,370 J m(-2)). After acellularization, the energy to break the valves decreased to 14,600 and 8,800 J m(-2) for the transgenic pulmonary valve and non-transgenic valve, respectively. The native transgenic pulmonary valve showed a higher extensibility (42.70 %) than the non-transgenic pulmonary valve (35.50 %); the extensibility decreased after acellularization to 41.1 and 31.5 % for the transgenic and non-transgenic valves, respectively. The pulmonary valves derived from transgenic pigs demonstrate better biomechanical properties compared with non-transgenic. Heart valves derived from transgenic pigs can be valuable for the preparation of tissue-engineered bioprostheses, because of their biomechanical properties, stability, reduced immune response, making them safer for clinical applications.
本研究的目的是评估源自转基因猪的心脏瓣膜管道的生物力学特性，以确定其在制备组织工程心脏瓣膜方面的实用性。使用来自转基因猪的去细胞主动脉和肺动脉瓣膜管道来评估瓣膜的生物力学特性。非转基因猪心脏瓣膜管道用作对照。无论是转基因猪还是非转基因猪的去细胞瓣膜管道，其生物力学稳定性均下降。与非转基因猪的天然肺动脉瓣膜（12,140±5,370 J m(-2)）相比，源自转基因猪的天然肺动脉瓣膜破裂所需能量更高（20,475±7,600 J m(-2)）。去细胞处理后，转基因肺动脉瓣膜和非转基因瓣膜破裂所需能量分别降至14,600和8,800 J m(-2)。天然转基因肺动脉瓣膜的延展性（42.70%）高于非转基因肺动脉瓣膜（35.50%）；去细胞处理后，转基因瓣膜和非转基因瓣膜的延展性分别降至41.1%和31.5%。与非转基因猪的瓣膜相比，源自转基因猪的肺动脉瓣膜表现出更好的生物力学特性。源自转基因猪的心脏瓣膜因其生物力学特性、稳定性、免疫反应降低，使其在临床应用中更安全，对于制备组织工程生物假体可能具有重要价值。
