Nafeesa Zohara, Shivalingu B R, Vivek H K, Priya B S, Swamy S Nanjunda
Department of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Technical Institutions Campus, Mysore, 570 006, Karnataka, India.
Appl Biochem Biotechnol. 2015 Mar;175(6):2787-94. doi: 10.1007/s12010-014-1462-5. Epub 2015 Jan 11.
Coagulation is an important physiological process in hemostasis which is activated by sequential action of proteases. This study aims to understand the involvement of aqueous fruit extract of Cucumis sativus L. (AqFEC) European burp less variety in blood coagulation cascade. AqFEC hydrolyzed casein in a dose-dependent manner. The presence of protease activity was further confirmed by casein zymography which revealed the possible presence of two high molecular weight protease(s). The proteolytic activity was inhibited only by phenyl methyl sulphonyl fluoride suggesting the presence of serine protease(s). In a dose-dependent manner, AqFEC also hydrolysed Aα and Bβ subunits of fibrinogen, whereas it failed to degrade the γ subunit of fibrinogen even at a concentration as high as 100 μg and incubation time up to 4 h. AqFEC reduced the clotting time of citrated plasma by 87.65%. The protease and fibrinogenolytic activity of AqFEC suggests its possible role in stopping the bleeding and ensuing wound healing process.
凝血是止血过程中的一个重要生理过程，由蛋白酶的顺序作用激活。本研究旨在了解欧洲无嗝品种黄瓜（Cucumis sativus L.）水提果实提取物（AqFEC）在血液凝固级联反应中的作用。AqFEC以剂量依赖的方式水解酪蛋白。酪蛋白酶谱进一步证实了蛋白酶活性的存在，该酶谱显示可能存在两种高分子量蛋白酶。蛋白水解活性仅被苯甲基磺酰氟抑制，表明存在丝氨酸蛋白酶。AqFEC还以剂量依赖的方式水解纤维蛋白原的Aα和Bβ亚基，而即使在浓度高达100μg且孵育时间长达4小时的情况下，它也未能降解纤维蛋白原的γ亚基。AqFEC使枸橼酸盐血浆的凝血时间缩短了87.65%。AqFEC的蛋白酶和纤维蛋白溶解活性表明其在止血和随后的伤口愈合过程中可能发挥作用。
