Alon Shahar, Eisenberg Eli
Department of Neurobiology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel.
Methods Mol Biol. 2015;1269:231-42. doi: 10.1007/978-1-4939-2291-8_14.
Deep sequencing has many possible applications; one of them is the identification and quantification of RNA editing sites. The most common type of RNA editing is adenosine to inosine (A-to-I) editing. A prerequisite for this editing process is a double-stranded RNA (dsRNA) structure. Such dsRNAs are formed as part of the microRNA (miRNA) maturation process, and it is therefore expected that miRNAs are affected by A-to-I editing. Indeed, tens of editing sites were found in miRNAs, some of which change the miRNA binding specificity. Here, we describe a protocol for the identification of RNA editing sites in mature miRNAs using deep sequencing data.
深度测序有许多可能的应用；其中之一是RNA编辑位点的识别和定量。最常见的RNA编辑类型是腺苷到肌苷（A-to-I）编辑。这种编辑过程的一个先决条件是双链RNA（dsRNA）结构。此类dsRNA是作为微小RNA（miRNA）成熟过程的一部分形成的，因此预计miRNA会受到A-to-I编辑的影响。事实上，在miRNA中发现了数十个编辑位点，其中一些改变了miRNA的结合特异性。在此，我们描述了一种使用深度测序数据识别成熟miRNA中RNA编辑位点的方法。
