用二十六烷酸（C26:0）处理的神经元SK-N-BE细胞中24S-羟基胆固醇的积累：支持24S-羟基胆固醇作为神经脂毒性潜在生物标志物的论据。

Accumulation of 24S-hydroxycholesterol in neuronal SK-N-BE cells treated with hexacosanoic acid (C26:0): argument in favor of 24S-hydroxycholesterol as a potential biomarker of neurolipotoxicity.

作者信息

Zarrouk A, Hammami M, Moreau T, Lizard G

机构信息

Team 'Biochemistry of Peroxisome, Inflammation and Lipid Metabolism', EA 7270, INSERM, faculté des sciences Gabriel, University of Bourgogne, 6, boulevard Gabriel, 21000 Dijon, France; LR12ES05, Lab-NAFS 'Nutrition-Functional Food and Vascular Health', faculté de médecine, University of Monastir, avenue Avicenne, Monastir, Tunisia.

LR12ES05, Lab-NAFS 'Nutrition-Functional Food and Vascular Health', faculté de médecine, University of Monastir, avenue Avicenne, Monastir, Tunisia.

出版信息

Rev Neurol (Paris). 2015 Feb;171(2):125-9. doi: 10.1016/j.neurol.2014.10.016. Epub 2015 Jan 8.

DOI:10.1016/j.neurol.2014.10.016

PMID:25577946

Abstract

Cholesterol oxide derivatives (oxysterols) are viewed as potential biomarkers of neurodegenerative diseases. 24S-hydroxycholesterol, an oxysterol produced only in brain neurons, is often found for unknown reasons in increased levels in the plasma in patients with neurodegenerative diseases. On human neuronal SK-N-BE cells treated with hexacosanoic acid (C26:0) identified at increased levels in the tissues and plasma of patients with peroxisomal leukodystrophies and Alzheimer's disease, we observed increased level of 24S-hydroxycholesterol associated with C26:0 induced lipotoxicity. This finding reinforces the hypothesis suggesting that 24S-hydroxycholesterol could constitute a biomarker of neurotoxicity.

摘要

胆固醇氧化衍生物（氧化甾醇）被视为神经退行性疾病的潜在生物标志物。24S-羟基胆固醇是一种仅在脑神经元中产生的氧化甾醇，在神经退行性疾病患者的血浆中，其水平常常会出于未知原因而升高。在过氧化物酶体脑白质营养不良症和阿尔茨海默病患者的组织和血浆中，二十六烷酸（C26:0）水平升高，在用其处理人神经元SK-N-BE细胞时，我们观察到与C26:0诱导的脂毒性相关的24S-羟基胆固醇水平升高。这一发现强化了如下假说，即24S-羟基胆固醇可能构成神经毒性的生物标志物。