Barteri Mario, De Carolis Roberta, Marinelli Fiorenzo, Tomassetti Goliardo, Montemiglio Linda Celeste
a Department of Chemistry , "Sapienza" University of Rome , Rome , Italy.
b National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) , Rome , Italy.
Electromagn Biol Med. 2016;35(2):126-33. doi: 10.3109/15368378.2014.1002135. Epub 2015 Jan 12.
This work shows the effects of exposure to an electromagnetic field at 900 MHz on the catalytic activity of the enzymes lactoperoxidase (LPO) and horseradish peroxidase (HRP). Experimental evidence that irradiation causes conformational changes of the active sites and influences the formation and stability of the intermediate free radicals is documented by measurements of enzyme kinetics, circular dichroism spectroscopy (CD) and cyclic voltammetry.
这项研究展示了暴露于900兆赫的电磁场对乳过氧化物酶(LPO)和辣根过氧化物酶(HRP)催化活性的影响。通过酶动力学测量、圆二色光谱法(CD)和循环伏安法记录的实验证据表明,辐射会导致活性位点的构象变化,并影响中间自由基的形成和稳定性。