Lee S M, Taguchi F
Department of Microbiology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
Arch Virol. 1989;108(3-4):247-57. doi: 10.1007/BF01310937.
A new enzyme-linked immunoassay system (TC-ELISA) was developed for the rapid, specific detection and typing of herpes simplex virus (HSV) using anti-HSV immune sera and enzyme-labeled complement component C1q (P*-C1q). The method is based on viral antigen produced in HSV-infected cells being detected by simultaneous addition of immune serum and P*-C1q (ELISA-CF). With this assay, it was found that HSV was detected with anti-HSV immune serum and P*-C1q and that HSV type 1 and HSV type 2 could be differentiated with anti-HSV-1 and anti-HSV-2 immune sera and P*-C1q. The TC-ELISA test was HSV specific and not reactive with measles virus, cytomegalovirus, mumps virus or varicella-zoster virus. The whole assay procedure can be completed within 3 hours, as long as HSV-inoculated cell cultures with more than 10% cytopathic effect are used. These results suggest that TC-ELISA is useful in the diagnosis of viral infections.
利用抗单纯疱疹病毒（HSV）免疫血清和酶标记补体成分C1q（P*-C1q），开发了一种新的酶联免疫分析系统（TC-ELISA），用于快速、特异性地检测和分型单纯疱疹病毒（HSV）。该方法基于通过同时加入免疫血清和P*-C1q（ELISA-CF）来检测HSV感染细胞中产生的病毒抗原。通过该检测方法发现，使用抗HSV免疫血清和P*-C1q可检测到HSV，并且使用抗HSV-1和抗HSV-2免疫血清及P*-C1q可区分1型HSV和2型HSV。TC-ELISA检测对HSV具有特异性，与麻疹病毒、巨细胞病毒、腮腺炎病毒或水痘-带状疱疹病毒无反应。只要使用具有超过10%细胞病变效应的HSV接种细胞培养物，整个检测过程可在3小时内完成。这些结果表明，TC-ELISA在病毒感染的诊断中是有用的。
