Li Jun, Wang Jing, Zhong Zhiyue, He Daikun, Zhang Jing, Shen Jie
Emergency & Intensive Care Centre for Chemical Accident of Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China.
Emergency & Intensive Care Centre for Chemical Accident of Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China. E-mail:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2014 Nov;32(11):813-8.
To investigate the dynamic changes of a group of cytokines in phosgene-induced lung injury and the function of different dose of ulinastatin through animal experiment.
104 male SD rats were randomly assigned into the control group, ulinastatin control group, phosgene treatment groups and different dose of ulinastatin intervention groups, 8 rats each group. Treatment groups were dynamic constant exposure in phosgene, and immediately injected ulinastatin intraperitoneal, and then the experimental animal, the lung tissue biopsy, lung wet/dry ratio, RT-PCR detection, the plasma for detection of Bio-Plex 18 cytokines.
Compared with the control group, plasma concentrations of IL-1α, IL-6, GM-CSF, TNF-α, INF-γ, MIP-3α, VEGF were increased significantly first (2 h), and gradually decreased with the passage of time , the difference was statistically significant (P < 0.05). Plasma concentrations of IL-4, IL-10 were decreased earlier (2h, 6 h) and increased later (24 h) (P < 0.05). The change of plasma concentration of IL-13 was not obvious earlier (2 h) and still rising later (24h), the difference was statistically significant (P < 0.05). After drug intervention, the levels of pro-inflammatory cytokines declined and the levels of anti-inflammatory cytokines raise by different degrees at different times in ulinastatin intervention groups, the difference was statistically significant. The degree of lung injury was improved than the phosgene treatment groups and better in high dose of ulinastatin intervention group. The expression of IL-10, IL-4, IL-13-mRNA of tissue increased in accordance with plasma results.
A group of cytokines are dynamicly changed in phosgene-induced lung injury by time. High dose of ulinastatin can improved phosgene-induced lung injury, regulate the synthesis and release of inflammatory cytokines and inhibit inflammatory react in a dose-dependent manner.
通过动物实验研究光气所致肺损伤中一组细胞因子的动态变化及不同剂量乌司他丁的作用。
将104只雄性SD大鼠随机分为对照组、乌司他丁对照组、光气处理组和不同剂量乌司他丁干预组，每组8只。处理组持续动态暴露于光气中，随即腹腔注射乌司他丁，然后对实验动物进行肺组织活检、测定肺湿/干比、RT-PCR检测，采集血浆检测Bio-Plex 18种细胞因子。
与对照组相比，血浆中IL-1α、IL-6、GM-CSF、TNF-α、INF-γ、MIP-3α、VEGF浓度首先显著升高（2小时），并随时间逐渐下降，差异有统计学意义（P<0.05）。IL-4、IL-10血浆浓度早期（2小时、6小时）下降，后期（24小时）升高（P<0.05）。IL-13血浆浓度早期（2小时）变化不明显，后期（24小时）仍在升高，差异有统计学意义（P<0.05）。药物干预后，乌司他丁干预组促炎细胞因子水平在不同时间不同程度下降，抗炎细胞因子水平升高，差异有统计学意义。肺损伤程度较光气处理组有所改善，高剂量乌司他丁干预组改善更明显。组织中IL-10、IL-4、IL-13-mRNA的表达与血浆结果一致。
光气所致肺损伤中一组细胞因子随时间动态变化。高剂量乌司他丁可改善光气所致肺损伤，调节炎症细胞因子的合成与释放，呈剂量依赖性抑制炎症反应。
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