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通过与葡聚糖进行美拉德型糖基化反应改善全荞麦蛋白组分的表面功能，包括降低致敏性。

Improvement of surface functionalities, including allergenicity attenuation, of whole buckwheat protein fraction by maillard-type glycation with dextran.

作者信息

Tazawa Shigeru, Katayama Shigeru, Hirabayashi Masahiro, Yamaguchi Daiki, Nakamura Soichiro

机构信息

Interdisciplinary Graduate School of Science and Technology, Shinshu University, Nagano 399-4598, Japan.

Interdisciplinary Graduate School of Science and Technology, Shinshu University, Nagano 399-4598, Japan ; Department of Agriculture, Shinshu University, Nagano 399-4598, Japan.

出版信息

Prev Nutr Food Sci. 2014 Dec;19(4):327-32. doi: 10.3746/pnf.2014.19.4.327. Epub 2014 Dec 31.

DOI:10.3746/pnf.2014.19.4.327
PMID:25580398
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4287326/
Abstract

The purpose of the current study was to determine the effects of the introduction of polysaccharide chains onto the molecular surface of buckwheat proteins on buckwheat protein surface functionality. The whole buckwheat protein fraction (WBP) was prepared using 50 mM phosphate buffer (pH 7.5) containing 0.5 M NaCl and covalently linked with 6 kDa, 17.5 kDa, 40 kDa, 70 kDa, or 200 kDa dextran by Maillard-type glycation through controlled dry-heating at 60°C and 79% relative humidity for two weeks. Conjugation with 40 kDa dextran improved the water solubility and emulsifying properties of WBP without causing a serious loss of available lysine; 84.9% of the free amino groups were conserved. In addition, we found that the introduction of dextran chains onto the molecular surfaces of WBP attenuated the antigenicity of WBP.

摘要

本研究的目的是确定在荞麦蛋白分子表面引入多糖链对荞麦蛋白表面功能的影响。使用含有0.5 M NaCl的50 mM磷酸盐缓冲液（pH 7.5）制备全荞麦蛋白组分（WBP），并通过在60°C和79%相对湿度下控制干热两周，通过美拉德型糖基化与6 kDa、17.5 kDa、40 kDa、70 kDa或200 kDa的葡聚糖共价连接。与40 kDa葡聚糖结合可提高WBP的水溶性和乳化性能，而不会导致可用赖氨酸的严重损失；84.9%的游离氨基得以保留。此外，我们发现将葡聚糖链引入WBP分子表面可减弱WBP的抗原性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/438e9e71a072/pnfs-19-327f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/3c4753e1d350/pnfs-19-327f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/86701e3572a5/pnfs-19-327f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/aaaa51035c3f/pnfs-19-327f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/31f7073108d3/pnfs-19-327f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/d383dfc34db6/pnfs-19-327f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/438e9e71a072/pnfs-19-327f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/3c4753e1d350/pnfs-19-327f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/86701e3572a5/pnfs-19-327f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/aaaa51035c3f/pnfs-19-327f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/31f7073108d3/pnfs-19-327f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/d383dfc34db6/pnfs-19-327f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a091/4287326/438e9e71a072/pnfs-19-327f6.jpg

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