Jambrosic J, Mancianti M L, Ricciardi R, Sela B A, Koprowski H, Herlyn M
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Int J Cancer. 1989 Dec 15;44(6):1117-23. doi: 10.1002/ijc.2910440630.
Infection of normal human melanocyte and nevus cultures with an adenovirus 12-Simian Virus 40 hybrid virus (Ad12-SV40) produced transformed cells that expressed SV40-T antigen. The Ad12-SV40 cells exhibited rapid cell proliferation to high cell densities and efficient growth in soft agar, but none of 15 transformed melanocyte and nevus cultures formed tumors when injected s.c. or under the renal capsule into athymic nude mice. While the Ad12-SV40-transformed cells lost certain properties associated with the melanocytic phenotype, i.e., pigmentation, tyrosinase activity and melanosome content, the expression of melanoma-associated antigens, including nerve growth factor receptor, p97 melano-transferrin, and chondroitin sulfate proteoglycan, remained stable. The transformed melanocytes acquired the ability to express HLA-DR antigen, which is found on nevus and melanoma cells. Total ganglioside patterns in Ad12-SV40-transformed cells changed to reflect more advanced stages of tumor progression. Transformed melanocytes, like nevus and melanoma cells, showed increased GD3 content and transformed nevus cells increased GD2 which is a feature of malignant melanoma cells. Ad12-SV40-transformed human melanocytes and nevus cells are useful tools for studying tumor progression under experimental conditions.
用腺病毒12-猴病毒40杂交病毒（Ad12-SV40）感染正常人黑素细胞和痣细胞培养物，产生了表达SV40-T抗原的转化细胞。Ad12-SV40细胞表现出快速的细胞增殖，可达到高细胞密度，并能在软琼脂中高效生长，但15种转化的黑素细胞和痣细胞培养物在皮下或肾被膜下注射到无胸腺裸鼠体内时均未形成肿瘤。虽然Ad12-SV40转化细胞丧失了某些与黑素细胞表型相关的特性，即色素沉着、酪氨酸酶活性和黑素体含量，但包括神经生长因子受体、p97黑素转铁蛋白和硫酸软骨素蛋白聚糖在内的黑色素瘤相关抗原的表达仍保持稳定。转化的黑素细胞获得了表达HLA-DR抗原的能力，该抗原存在于痣细胞和黑色素瘤细胞上。Ad12-SV40转化细胞中的总神经节苷脂模式发生变化，以反映肿瘤进展的更晚期阶段。转化的黑素细胞，如痣细胞和黑色素瘤细胞，显示GD3含量增加，转化的痣细胞GD2增加，这是恶性黑色素瘤细胞的一个特征。Ad12-SV40转化的人黑素细胞和痣细胞是在实验条件下研究肿瘤进展的有用工具。
