Ono T, Ota A, Ito K, Nakaoka T, Karnan S, Konishi H, Furuhashi A, Hayashi T, Yamada Y, Hosokawa Y, Kazaoka Y
Department of Oral and Maxillofacial Surgery, Aichi Medical University Hospital, Nagakute, Japan.
Oral Dis. 2015 May;21(4):501-11. doi: 10.1111/odi.12310. Epub 2015 Jan 29.
Plumbagin (PL), a naturally occurring quinoid, exerts antitumoral effects in diverse types of cancer cells. However, the effect of PL on tumor cell proliferation in oral squamous cell carcinoma (OSCC) remains poorly understood. In this study, we assessed the efficacy of PL, in human OSCC cells.
The effect of PL on the cell growth and apoptosis of OSCC cell lines was evaluated using MTT and Annexin V assays, respectively. The effect of PL on mitochondrial membrane potential loss and reactive oxygen species (ROS) generation was evaluated using flow cytometry analysis.
MTT assay showed that PL dose-dependently suppressed OSCC cell growth, with IC50 values ranging from 3.87 to 14.6 μM. Flow cytometry analysis revealed that PL treatment resulted in a significant decrease in mitochondrial membrane potential and an increase in the number of apoptotic cells. Notably, ROS generation was significantly elevated after PL treatment. Furthermore, a ROS scavenger, N-acetylcysteine (NAC), clearly suppressed the decrease in mitochondrial membrane potential, increase of caspase-3/7 activity, and apoptosis after PL treatment.
This study provides the considerable evidence of the tumor-suppressive effect of PL, thereby highlighting its therapeutic potential for OSCC treatment.
白花丹醌（PL）是一种天然存在的醌类化合物，对多种癌细胞具有抗肿瘤作用。然而，PL对口腔鳞状细胞癌（OSCC）肿瘤细胞增殖的影响仍知之甚少。在本研究中，我们评估了PL对人OSCC细胞的疗效。
分别使用MTT和Annexin V检测法评估PL对OSCC细胞系细胞生长和凋亡的影响。使用流式细胞术分析评估PL对线粒体膜电位丧失和活性氧（ROS）生成的影响。
MTT检测显示，PL剂量依赖性地抑制OSCC细胞生长，IC50值范围为3.87至14.6 μM。流式细胞术分析表明，PL处理导致线粒体膜电位显著降低，凋亡细胞数量增加。值得注意 的是，PL处理后ROS生成显著升高。此外，一种ROS清除剂N-乙酰半胱氨酸（NAC）明显抑制了PL处理后线粒体膜电位的降低、caspase-3/7活性的增加和凋亡。
本研究提供了大量证据证明PL的肿瘤抑制作用，从而突出了其在OSCC治疗中的治疗潜力。
