Blood and Transplantation Center of Coimbra, Portuguese Institute of the Blood and Transplantation, Quinta da Vinha Moura, São Martinho do Bispo, 3041-861 Coimbra, Portugal.
College of Health Technology of Coimbra, Rua 5 de Outubro, 3046-854 Coimbra, Portugal; Centro Hospitalar Tondela-Viseu, EPE, Av. Rei D. Duarte, 2504-509 Viseu, Portugal.
Leuk Res. 2015 Mar;39(3):361-70. doi: 10.1016/j.leukres.2014.12.009. Epub 2014 Dec 24.
Erythroid dysplasia is a common feature of myelodysplastic syndromes (MDS). Currently available information about the immunophenotypic features of normal and dysplastic erythropoiesis is scarce and restricted to relatively few markers. Here we studied the expression of CD117, CD35 and CD44 throughout the normal (n=16) and dysplastic (n=48) bone marrow erythroid maturation. CD35 emerged as an early marker of CD34(+) erythroid-committed precursors, which is expressed before CD105 and remains positive thereafter. MDS patients (with and without morphologic dyserythropoiesis) displayed overall increased expression of CD44, associated with slight alterations on CD35 expression, suggesting that phenotypic alterations in MDS may precede morphologic dysplasia. In turn, MDS patients with anemia showed increased expression of CD117.
红细胞病态造血是骨髓增生异常综合征（MDS）的一个常见特征。目前关于正常和病态造血的免疫表型特征的信息很少，且仅限于相对较少的标记物。在这里，我们研究了 CD117、CD35 和 CD44 在正常（n=16）和病态（n=48）骨髓红系成熟过程中的表达。CD35 是 CD34(+)红系定向祖细胞的早期标记物，在 CD105 之前表达，并在此后保持阳性。MDS 患者（有无形态学红细胞发育异常）表现出 CD44 的总体表达增加，同时伴有 CD35 表达的轻微改变，提示 MDS 中的表型改变可能先于形态学发育不良。反过来，贫血的 MDS 患者 CD117 的表达增加。
