Luo X G, Hevner R F, Wong-Riley M T
Department of Anatomy and Cellular Biology, Medical College of Wisconsin, Milwaukee 53226.
J Neurosci Methods. 1989 Dec;30(3):189-95. doi: 10.1016/0165-0270(89)90129-5.
The relationship between the levels of cytochrome oxidase and gamma-aminobutyric acid (GABA) was investigated within single neurons by double labeling the 2 markers in the same section. Double staining was equally effective when immunogold-silver staining of GABA was followed by indirect immunoperoxidase labeling of cytochrome oxidase, or when cytochrome oxidase histochemistry was followed by immunogold-silver staining of GABA. Neurons in the perigeniculate nucleus (PGN) and basket cell terminals in the cerebellum were GABA positive and rich in cytochrome oxidase. Interneurons of the lateral geniculate nucleus (LGN) as well as stellate and Golgi cells of the cerebellum were GABA-rich but poor in cytochrome oxidase. These results demonstrate that there is no consistent relationship between the levels of cytochrome oxidase and GABA in neurons.
通过在同一切片中对两种标志物进行双重标记，研究了单个神经元内细胞色素氧化酶水平与γ-氨基丁酸（GABA）之间的关系。当对GABA进行免疫金银染色后再对细胞色素氧化酶进行间接免疫过氧化物酶标记，或者先进行细胞色素氧化酶组织化学染色再对GABA进行免疫金银染色时，双重染色同样有效。膝周核（PGN）中的神经元以及小脑中的篮状细胞终末GABA呈阳性且富含细胞色素氧化酶。外侧膝状核（LGN）的中间神经元以及小脑的星状细胞和高尔基细胞富含GABA但细胞色素氧化酶含量较低。这些结果表明，神经元中细胞色素氧化酶水平与GABA之间不存在一致的关系。
