Birkenbihl R P, Vielmetter W
Institut für Genetik, Universität zu Köln, Federal Republic of Germany.
Mol Gen Genet. 1989 Dec;220(1):147-53. doi: 10.1007/BF00260869.
In this paper complete distribution maps are presented of the seven IS elements 1, 2, 3, 4, 5, 30 and 150. These maps were obtained during the construction of an almost complete restriction map of the Escherichia coli genome of K12 strain BHB2600. The positions of IS elements were correlated to this map. The distribution of integration sites of all IS types is nonrandom. Besides a large gap from 79 min to 96 min, there is a pronounced IS cluster at 6 min and another at 97 min, map locations that have low gene incidences on the classical map. One cluster coincides with a region of IS induced rearrangements. The IS distribution pattern was compared to patterns of strains W3110 and HB101.
本文给出了7种插入序列（IS），即IS1、IS2、IS3、IS4、IS5、IS30和IS150的完整分布图。这些图是在构建K12菌株BHB2600大肠杆菌基因组的近乎完整的限制酶切图谱过程中获得的。插入序列的位置与该图谱相关联。所有类型插入序列的整合位点分布并非随机。除了从79分钟到96分钟有一个大的间隔外，在6分钟处有一个明显的插入序列簇，在97分钟处还有一个，这些图谱位置在经典图谱上基因发生率较低。其中一个簇与插入序列诱导重排的区域重合。将该插入序列分布模式与W3110菌株和HB101菌株的模式进行了比较。
