Fauler J, Marx K H, Kaever V, Frölich J C
Department of Clinical Pharmacology, Medical School Hannover, West-Germany.
Prostaglandins Leukot Essent Fatty Acids. 1989 Sep;37(3):193-6. doi: 10.1016/0952-3278(89)90085-9.
Human monocytes metabolize LTB4 by an additional pathway different from omega-oxidation. Reverse-phase high performance liquid chromatography showed four metabolites: 20-COOH-LTB4, 20-OH-LTB4 and two metabolites less polar than LTB4 with an UV maximum at 232 nm. Gas-chromatography mass-spectrometry showed nearly identical mass spectra for both metabolites. The main mass fragments of the two metabolites were increased by two mass units compared to LTB4. Our findings suggest that LTB4 had been reduced to a known and a new dihydro-metabolite of LTB4. Both metabolites together amounted to 85% of total metabolites. The remaining 15% were omega-oxidation products. Thus, the major pathway of LTB4 metabolism by human monocytes is reduction to dihydro-LTB4.
人类单核细胞通过一条不同于ω-氧化的额外途径代谢白三烯B4（LTB4）。反相高效液相色谱显示有四种代谢产物：20-羧基-LTB4、20-羟基-LTB4以及两种极性小于LTB4且在232nm处有最大紫外吸收的代谢产物。气相色谱-质谱分析显示这两种代谢产物的质谱几乎相同。与LTB4相比，这两种代谢产物的主要质谱碎片增加了两个质量单位。我们的研究结果表明，LTB4已被还原为一种已知的和一种新的LTB4二氢代谢产物。这两种代谢产物合计占总代谢产物的85%。其余15%是ω-氧化产物。因此，人类单核细胞代谢LTB4的主要途径是还原为二氢-LTB4。
