Friedrich P, Gallyas F
Institute of Enzymology, Hungarian Academy of Sciences, Budapest.
Acta Biochim Biophys Hung. 1989;24(1-2):61-8.
The protein synthesis in dissected whole larval brains of Drosophila melanogaster has been monitored by [35S] methionine incorporation, as revealed by two-dimensional gel-electrophoresis and fluorography. In wild type brains, drugs known to increase cAMP level increased the labelling of at least two proteins in the Mr range 30 to 120 kD and pI range 4.8 to 6.2. One of these proteins, Mr = 78 kD and pI = 5.9, was also enhanced in the dunceM11 memory-mutant, which has an elevated cAMP level, whereas it was hardly affected in the rutabaga memory-mutant, which has a subnormal cAMP level. It is suggested that cAMP-induced alterations in protein composition and/or turnover of nerve cells may contribute to the development of memory deficit in the dunce strains.
通过[35S]甲硫氨酸掺入法监测黑腹果蝇全幼虫脑的蛋白质合成，二维凝胶电泳和荧光自显影显示了这一过程。在野生型大脑中，已知能提高cAMP水平的药物增加了至少两种蛋白质的标记，其分子量在30至120 kD范围内，等电点在4.8至6.2范围内。其中一种蛋白质，分子量为78 kD，等电点为5.9，在cAMP水平升高的“笨蛋”M11记忆突变体中也有所增强，而在cAMP水平低于正常的“芜菁甘蓝”记忆突变体中几乎没有受到影响。有人认为，cAMP诱导的神经细胞蛋白质组成和/或周转的改变可能导致“笨蛋”品系记忆缺陷的发展。
