Chen Xiang, Feng Jiake, Ge Zhijun, Chen Hong, Ding Weiliang, Zhu Wenjiao, Tang Xiaoyan, Chen Yanyu, Tan Yongfei, Ma Tieliang
Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
Department of Critical Care Medicine, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
Mol Med Rep. 2015 May;11(5):3848-53. doi: 10.3892/mmr.2015.3188. Epub 2015 Jan 13.
The present study aimed to investigate whether the JWA gene can regulate the proliferation, migration and invasion of human breast cancer cells through the MAPK signaling pathway. The role of JWA in proliferation, migration, invasion and apoptosis was investigated in the MDA‑MB‑231 human breast cancer cell line. Following transfection with JWA‑small interfering (si)RNA, the effect of JWA on apoptosis was assessed by Western blot analysis, proliferation was determined using Transwell chambers and cell migration and invasion were analyzed by transwell assay. The expression levels of extracellular signal‑regulated kinase (ERK) 1/2, CSBP/RK/Mpk2 kinase (p38) and c‑Jun N‑terminal kinase (JNK) were detected using Western blot analysis in the siRNA and control groups. The expression of JWA in the breast cancer cells was significantly lower compared with the normal breast cells. Downregulation of JWA protein levels reduced the apoptosis and enhanced proliferation, migration and invasion of the MDA‑MB‑231 cells in vitro. The results of the Western blot analysis demonstrated that, compared with the control groups, the expression levels of phosphorylated (p‑)p38 decreased significantly in the JWA siRNA group. No significant changes were observed in the expression levels of p‑ERK1/2 or p‑JNK. Therefore, the JWA gene may regulate human breast cancer cells through the MAPK signaling pathway using different types of regulation.
本研究旨在探讨JWA基因是否能通过丝裂原活化蛋白激酶（MAPK）信号通路调节人乳腺癌细胞的增殖、迁移和侵袭。在MDA-MB-231人乳腺癌细胞系中研究了JWA在增殖、迁移、侵袭和凋亡中的作用。用JWA小干扰（si）RNA转染后，通过蛋白质印迹分析评估JWA对凋亡的影响，使用Transwell小室测定增殖，并通过Transwell试验分析细胞迁移和侵袭。在siRNA组和对照组中，使用蛋白质印迹分析检测细胞外信号调节激酶（ERK）1/2、CSBP/RK/Mpk2激酶（p38）和c-Jun氨基末端激酶（JNK）的表达水平。与正常乳腺细胞相比，乳腺癌细胞中JWA的表达显著降低。JWA蛋白水平的下调减少了体外MDA-MB-231细胞的凋亡，并增强了其增殖、迁移和侵袭。蛋白质印迹分析结果表明，与对照组相比，JWA siRNA组中磷酸化（p-）p38的表达水平显著降低。p-ERK1/2或p-JNK的表达水平未观察到显著变化。因此，JWA基因可能通过MAPK信号通路以不同类型的调节方式调控人乳腺癌细胞。
