Parsaei Parvin, Bahmaei Manouchehr, Ghannadi AliReza
Department of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, Iran.
School of Pharmacy and Pharmaceutical Sciences and Isfahan Pharmaceutical Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Iran J Pharm Res. 2014 Fall;13(4):1437-45.
Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile phase consisted of 8% methanol and 92% aqueous phase (1% (V/V) acetic acid water solution); flow rate, 0.7 mL/min; detection wavelength, 260 nm and pH, 3.3. The extraction efficiency of thiamine, nicotinamide and pyridoxine was influenced by factors such as: additional salt effect, the kind and volume of disperser and extraction solvents. In this research, the limit of detection (LOD) and quantification (LOQ) were 0.9 and 3 ng/mL for thiamine, 1.5 and 5 ng/mL for nicotinamide, 0.9 and 3 ng/mL for pyridoxine. The relative standard deviations (RSDs) were less than 2.87% (n=3). An appropriate linear behavior over the observed concentration range was obtained with the value of R²>0.996 for the target vitamins. This method was successfully applied to the sour cherry juice samples. Sour cherry var. Gise (Prunus cerasus var. Gise), which was used in this research, was a local variety of the sour cherry with large stone, double flowers, double fruits, dark red skin and dark red juice. This variety was identified in high altitude areas of Isfahan province after five years of study, since 2005, by Agricultural and Natural Resources Research Center of Isfahan.
采用分散液液微萃取法（DLLME）结合高效液相色谱-紫外检测法（HPLC-UV）测定酸樱桃汁中的硫胺素（B1）、烟酰胺（B3）和吡哆醇（B6）。该方法快速、简便且灵敏。使用C18柱进行分离。最佳色谱条件为：流动相由8%甲醇和92%水相（1%（V/V）乙酸水溶液）组成；流速为0.7 mL/min；检测波长为260 nm，pH值为3.3。硫胺素、烟酰胺和吡哆醇的萃取效率受以下因素影响：额外的盐效应、分散剂和萃取溶剂的种类及体积。本研究中，硫胺素的检测限（LOD）和定量限（LOQ）分别为0.9和3 ng/mL，烟酰胺为1.5和5 ng/mL，吡哆醇为0.9和3 ng/mL。相对标准偏差（RSDs）小于2.87%（n = 3）。目标维生素在观察到的浓度范围内呈现出合适的线性关系，R²>0.996。该方法成功应用于酸樱桃汁样品。本研究中使用的酸樱桃品种Gise（欧洲酸樱桃变种Gise）是一种当地的酸樱桃品种，果核大，花重瓣，果实重瓣，果皮深红色，果汁深红色。自2005年起，经过五年研究，该品种在伊斯法罕省的高海拔地区被伊斯法罕农业和自然资源研究中心鉴定出来。
