一项关于传染性法氏囊病病毒参考血清的国际合作研究。

An international collaborative study of an anti-infectious bursal disease virus reference serum.

作者信息

Reed N E, Thornton D H, Hebert C N, Muskett J C, Wood G W

机构信息

Central Veterinary Laboratory, Weybridge, Surrey, England.

出版信息

J Biol Stand. 1989 Oct;17(4):361-9. doi: 10.1016/s0092-1157(89)80007-1.

DOI:10.1016/s0092-1157(89)80007-1

PMID:2559083

Abstract

Fourteen laboratories participated in a collaborative study of a freeze-dried preparation of anti-infectious bursal disease virus serum to assess the suitability of the serum as a standard for use in the infectious bursal disease virus neutralization test. Ten laboratories carried out micro-virus neutralization tests and six carried out plaque reduction tests, two laboratories carrying out both tests. When titres were expressed as a proportion of that obtained for a reference preparation there was a marked reduction in variation between results from different laboratories. The use of a reference preparation was therefore of value when comparing results from different laboratories. It is proposed that the reference preparation used in this study be used as a standard to facilitate the comparison of results from different laboratories. The proposed standard contains by definition 10,000 UK units.

摘要

14个实验室参与了一项关于抗传染性法氏囊病病毒血清冻干制剂的协作研究，以评估该血清作为传染性法氏囊病病毒中和试验标准品的适用性。10个实验室进行了微量病毒中和试验，6个实验室进行了蚀斑减少试验，2个实验室同时进行了这两种试验。当效价表示为参考制剂效价的比例时，不同实验室结果之间的差异显著降低。因此，在比较不同实验室的结果时，使用参考制剂是有价值的。建议将本研究中使用的参考制剂用作标准品，以方便比较不同实验室的结果。根据定义，拟议的标准品含有10,000个英国单位。