Ozasa S, Kempner E S, Erickson S K
Division of Gastroenterology, Stanford University School of Medicine, CA 94305.
J Lipid Res. 1989 Nov;30(11):1759-62.
Rat liver acyl coenzyme A:diacylglycerol acyltransferase, an intrinsic membrane activity associated with the endoplasmic reticulum, catalyzes the terminal and rate-limiting step in triglyceride synthesis. This enzyme has never been purified nor has its gene been isolated. Inactivation by ionizing radiation and target analysis were used to determine its functional size in situ. Monoexponential radiation inactivation curves were obtained which indicated that a single-sized unit of 72 +/- 4 kDa is required for expression of activity. The size corresponds only to the protein portion of the target and may represent one or several polypeptides.
大鼠肝脏酰基辅酶A：二酰甘油酰基转移酶是一种与内质网相关的内在膜活性物质，催化甘油三酯合成的终末限速步骤。该酶从未被纯化，其基因也未被分离。利用电离辐射灭活和靶点分析来原位确定其功能大小。获得了单指数辐射灭活曲线，表明活性表达需要一个大小为72±4 kDa的单一单位。该大小仅对应于靶点的蛋白质部分，可能代表一种或几种多肽。
