Lee S K, Oh K H, Chung A Y, Park H C, Lee S H, Kwon S Y, Choi J
Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Republic of Korea.
Laboratory of Neurodevelopmental Genetics, Graduate School of Medicine, Korea University, Ansan, Republic of Korea.
Hum Exp Toxicol. 2015 Nov;34(11):1043-52. doi: 10.1177/0960327114567766. Epub 2015 Jan 14.
The aim of this study was to evaluate the protective effects of quercetin on cisplatin-induced hair cell damage in transgenic zebrafish embryos.
Five days postfertilization zebrafish embryos were exposed to 1 mM cisplatin and quercetin at 10, 50, 100, or 200 μM for 4 h. Hair cells within neuromasts of the supraorbital, otic, and occipital lateral lines were analyzed by fluorescent microscopy (n = 10). Survival of hair cells was calculated as the average number of hair cells in the control group that were not exposed to cisplatin. Ultrastructural changes were evaluated using scanning electron microscopy.
Hair cell damage in neuromasts was decreased by co-treatment of quercetin and cisplatin (quercetin 100 μM: 8.6 ± 1.1 cells; 1 mM cisplatin only: 5.0 ± 0.5 cells; n = 10, p < 0.05); apoptosis of hair cells examined by special stain was also decreased by quercetin. The ultrastructure of hair cells within neuromasts was preserved in zebrafish by the combination of quercetin (100 μM) and cisplatin (1 mM).
In conclusion, quercetin showed protective effects against cisplatin-induced toxicity in a zebrafish model. The results of this study suggest the possibility of a protective role of quercetin against cisplatin-induced apoptotic cell death in zebrafish.
本研究旨在评估槲皮素对顺铂诱导的转基因斑马鱼胚胎毛细胞损伤的保护作用。
受精后5天的斑马鱼胚胎暴露于1 mM顺铂以及10、50、100或200 μM的槲皮素中4小时。通过荧光显微镜分析眶上、耳和枕侧线神经丘内的毛细胞（n = 10）。毛细胞存活率以未暴露于顺铂的对照组毛细胞平均数计算。使用扫描电子显微镜评估超微结构变化。
槲皮素与顺铂联合处理可减少神经丘内的毛细胞损伤（槲皮素100 μM：8.6 ± 1.1个细胞；仅1 mM顺铂：5.0 ± 0.5个细胞；n = 10，p < 0.05）；通过特殊染色检测的毛细胞凋亡也因槲皮素而减少。槲皮素（100 μM）与顺铂（1 mM）联合可使斑马鱼神经丘内毛细胞的超微结构得以保存。
总之，在斑马鱼模型中，槲皮素对顺铂诱导的毒性具有保护作用。本研究结果提示槲皮素对斑马鱼中顺铂诱导的凋亡性细胞死亡具有保护作用的可能性。
