氟奋乃静：从孤立分子到其与脂质双层的相互作用

Fluphenazine: from an isolated molecule to its interaction with lipid bilayers.

作者信息

Petrus Joanna, Czarnik-Matusewicz Bogusława, Petrus Rafał, Cieślik-Boczula Katarzyna, Jaszczyszyn Agata, Gąsiorowski Kazimierz

机构信息

Department of Chemistry, University of Wrocław, F. Joliot Curie 14, Wrocław 50-383, Poland.

出版信息

Chem Phys Lipids. 2015 Feb;186:51-60. doi: 10.1016/j.chemphyslip.2015.01.002. Epub 2015 Jan 13.

DOI:10.1016/j.chemphyslip.2015.01.002

PMID:25595294

Abstract

Fluphenazine (FPh) belongs to the phenothiazine family of compounds and exhibits a wide variety of biological effects, including antimutagenic, proapoptotic, antiproliferative and anti-multidrug resistance (MDR) activities. The ability of FPh to interact with lipid membranes can have a significant impact on its biological activities. However, the mechanisms involved in the interaction of FPh with lipid membranes are poorly understood. FTIR-ATR spectroscopy has been used in this study to visualize the interactions between FPh and a model lipid bilayer composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Subsequent interpretation of the temperature-dependent FTIR spectra obtained for FPh:DPPC systems containing different concentrations of FPh was efficiently supported by principal component analysis.

摘要

氟奋乃静（FPh）属于吩噻嗪类化合物，具有多种生物学效应，包括抗诱变、促凋亡、抗增殖和抗多药耐药（MDR）活性。FPh与脂质膜相互作用的能力对其生物学活性可能有重大影响。然而，FPh与脂质膜相互作用的机制尚不清楚。本研究采用傅里叶变换红外衰减全反射光谱（FTIR-ATR）来观察FPh与由1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）组成的模型脂质双层之间的相互作用。主成分分析有效地支持了对含有不同浓度FPh的FPh:DPPC体系获得的温度依赖性FTIR光谱的后续解释。