流式细胞术分析淋巴样增强子结合因子1在慢性淋巴细胞白血病/小淋巴细胞淋巴瘤诊断中的应用

Flow cytometric analysis of lymphoid enhancer-binding factor 1 in diagnosis of chronic lymphocytic leukemia/small lymphocytic lymphoma.

作者信息

Amador-Ortiz Catalina, Goolsby Charles L, Peterson LoAnn C, Wolniak Kristy L, McLaughlin Janet L, Gao Juehua, Chen Yi-Hua

机构信息

From the Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL.

From the Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL. From the Department of Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL.

出版信息

Am J Clin Pathol. 2015 Feb;143(2):214-22. doi: 10.1309/AJCPT32JDFIGHFHJ.

DOI:10.1309/AJCPT32JDFIGHFHJ

PMID:25596247

Abstract

OBJECTIVES

Nuclear overexpression of lymphoid enhancer-binding factor 1 (LEF1) assessed by immunohistochemistry has been shown to be highly associated with chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) among small B-cell lymphomas. The purpose of this study was to evaluate the utility of flow cytometric analysis of LEF1 in the diagnosis of CLL/SLL.

METHODS

Normal peripheral blood was used to validate the test. Flow cytometric analysis of LEF1 was performed in 64 patient samples qualitatively and quantitatively by comparing the staining intensity and the ratios of the median fluorescence intensities (MFIs) of LEF1 in B cells of interest to the internal reference cell populations. The results were correlated with the pathologic diagnosis.

RESULTS

Proper sample processing ensured sufficient separation of positive LEF1 staining in T cells from negative staining in normal B and natural killer (NK) cells. Qualitative analysis of patient samples showed that all 25 cases of CLL/SLL but none of the other small B-cell lymphomas were positive for LEF1. Using a B/NK MFI ratio of 1.5 and B/T MFI ratio of 0.45 separated CLL/SLL cases from non-CLL lymphomas.

CONCLUSIONS

Flow cytometric analysis of LEF1 is sufficient to differentiate CLL/SLL from other small B-cell lymphomas and may serve as a useful tool in the diagnosis of CLL/SLL.

摘要

目的

免疫组织化学评估显示，在小B细胞淋巴瘤中，淋巴样增强因子1（LEF1）的核过表达与慢性淋巴细胞白血病/小淋巴细胞淋巴瘤（CLL/SLL）高度相关。本研究的目的是评估LEF1的流式细胞术分析在CLL/SLL诊断中的实用性。

方法

使用正常外周血验证该检测方法。通过比较感兴趣的B细胞中LEF1的染色强度和中位荧光强度（MFI）与内部参考细胞群的比率，对64例患者样本进行LEF1的流式细胞术定性和定量分析。结果与病理诊断相关。

结果

适当的样本处理确保了T细胞中LEF1阳性染色与正常B细胞和自然杀伤（NK）细胞中的阴性染色充分分离。对患者样本的定性分析显示，所有25例CLL/SLL均为LEF1阳性，而其他小B细胞淋巴瘤均为阴性。使用B/NK MFI比率为1.5和B/T MFI比率为0.45可将CLL/SLL病例与非CLL淋巴瘤区分开来。