Li Xiang Z, Gao Qing S, Yan Chang G, Choi Seong H, Shin Jong S, Song Man K
Department of Animal Science, Yanbian University, Yanji, China.
Department of Animal Science, Chungbuk National University, Cheong-ju, Chungbuk, Korea.
Anim Sci J. 2015 Aug;86(8):755-64. doi: 10.1111/asj.12354. Epub 2015 Jan 19.
We hypothesized that manipulating metabolism with fish oil and malate as a hydrogen acceptor would affect the biohydrogenation process of α-linolenic acid by rumen microbes. This study was to examine the effect of fish oil and/or malate on the production of conjugated fatty acids and methane (CH4 ) by rumen microbes when incubated with linseed oil. Linseed oil (LO), LO with fish oil (LO-FO), LO with malate (LO-MA), or LO with fish oil and malate (LO-FO-MA) was added to diluted rumen fluid, respectively. The LO-MA and LO-FO-MA increased pH and propionate concentration compared to the other treatments. LO-MA and LO-FO-MA reduced CH4 production compared to LO. LO-MA and LO-FO-MA increased the contents of c9,t11-conjugated linoleic acid (CLA) and c9,t11,c15-conjugated linolenic acid (CLnA) compared to LO. The content of malate was rapidly reduced while that of lactate was reduced in LO-MA and LO-FO-MA from 3 h incubation time. The fold change of the quantity of methanogen related to total bacteria was decreased at both 3 h and 6 h incubation times in all treatments compared to the control. Overall data indicate that supplementation of combined malate and/or fish oil when incubated with linseed oil, could depress methane generation and increase production of propionate, CLA and CLnA under the conditions of the current in vitro study.
我们假设，以鱼油和苹果酸作为氢受体来调控新陈代谢，会影响瘤胃微生物对α-亚麻酸的生物氢化过程。本研究旨在考察当与亚麻籽油一起培养时，鱼油和/或苹果酸对瘤胃微生物共轭脂肪酸和甲烷（CH4）产生的影响。分别将亚麻籽油（LO）、含鱼油的亚麻籽油（LO-FO）、含苹果酸的亚麻籽油（LO-MA）或含鱼油和苹果酸的亚麻籽油（LO-FO-MA）添加到稀释的瘤胃液中。与其他处理相比，LO-MA和LO-FO-MA提高了pH值和丙酸盐浓度。与LO相比，LO-MA和LO-FO-MA降低了CH4的产生。与LO相比，LO-MA和LO-FO-MA增加了c9,t11-共轭亚油酸（CLA）和c9,t11,c15-共轭亚麻酸（CLnA）的含量。在培养3小时后，LO-MA和LO-FO-MA中苹果酸的含量迅速降低，而乳酸的含量也有所降低。与对照相比，在所有处理的3小时和6小时培养时间点，与总细菌相关的产甲烷菌数量的变化倍数均降低。总体数据表明，在当前体外研究条件下，当与亚麻籽油一起培养时，添加苹果酸和/或鱼油可抑制甲烷生成，并增加丙酸盐、CLA和CLnA的产生。
