LeVatte M A, Sokol P A
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.
J Antimicrob Chemother. 1989 Dec;24(6):881-95. doi: 10.1093/jac/24.6.881.
Growth of Pseudomonas aeruginosa in medium containing sub-inhibitory concentrations of tobramycin, tetracycline or chloramphenicol repressed surface expression of the ferripyochelin binding protein (FBP). Ciprofloxacin did not repress FBP surface expression but increased the amount of detectable FBP. Sub-MICs of tetracycline, tobramycin and chloramphenicol also reduced ferripyochelin uptake by whole cells. An additional Mr = 19,000 protein was detected with monoclonal antibody in outer membranes of cultures grown in the presence of tetracycline and chloramphenicol. This protein is presumed to be a precursor form of FBP. No other major changes in LPS migration patterns or outer membrane protein profiles were observed in cultures grown in the presence of these antibiotics. These data suggest that exposure of P. aeruginosa to sublethal doses of tobramycin, tetracycline or chloramphenicol can alter the ability of these organisms to acquire iron.
在含有亚抑制浓度妥布霉素、四环素或氯霉素的培养基中，铜绿假单胞菌的生长抑制了铁载体结合蛋白（FBP）的表面表达。环丙沙星不抑制FBP表面表达，但增加了可检测到的FBP量。四环素、妥布霉素和氯霉素的亚抑菌浓度也降低了全细胞对铁载体的摄取。用单克隆抗体在四环素和氯霉素存在下培养的细菌外膜中检测到一种额外的分子量为19,000的蛋白质。这种蛋白质被推测为FBP的前体形式。在这些抗生素存在下培养的细菌中，未观察到脂多糖迁移模式或外膜蛋白谱的其他主要变化。这些数据表明，铜绿假单胞菌暴露于亚致死剂量的妥布霉素、四环素或氯霉素会改变这些细菌获取铁的能力。
