Umeda Y, Arisawa M
Department of Chemotherapy and Biochemistry, Nippon Roche Research Center, Kamakura, Japan.
Immunopharmacol Immunotoxicol. 1989;11(2-3):309-20. doi: 10.3109/08923978909005372.
The effect of calcitonin gene-related peptide (CGRP) on natural killer (NK) cell activity in spleen cells from Balb/c mice and nude mice was studied. CGRP dose-dependently (10(-9) to 10(-7) M) inhibited NK activity of spleen cells from both strains of mice. This inhibitory effect was observed at the effector to target ratios of 12.5:1 to 100:1. Maximum inhibition by 10(-7) M CGRP was about 60%. The inhibition of NK activity by CGRP was also observed in anti-Thy 1.2 plus complement treated Balb/c spleen cells. Furthermore, when cells were treated with 10(-9) to 10(-7) M CGRP the concentration of intracellular cyclic AMP increased in spleen cells of nude mice. The characteristics of these cells were similar to those of NK cells, (1) being petri dish and nylon wool nonadherent, (2) expressing asialo GM1 antigen, and (3) lacking readily detectable Thy 1 antigen and immunoglobulin. In addition, the intravenous injection of asialo GM1 completely abolished NK activity in spleen cells from nude mice and the increase in intracellular cyclic AMP in spleen cells by CGRP was less in spleen cells from mice given an anti-asialo GM1 injection. Our present study suggests that CGRP inhibits NK cell activity by increasing the intracellular cyclic AMP concentration. CGRP may be implicated in the regulation of NK function.
研究了降钙素基因相关肽（CGRP）对Balb/c小鼠和裸鼠脾细胞中自然杀伤（NK）细胞活性的影响。CGRP呈剂量依赖性（10⁻⁹至10⁻⁷M）抑制两种品系小鼠脾细胞的NK活性。在效应细胞与靶细胞比例为12.5:1至100:1时观察到这种抑制作用。10⁻⁷M CGRP的最大抑制率约为60%。在抗Thy 1.2加补体处理的Balb/c脾细胞中也观察到CGRP对NK活性的抑制作用。此外，当用10⁻⁹至10⁻⁷M CGRP处理细胞时，裸鼠脾细胞内细胞内环磷酸腺苷（cAMP）浓度增加。这些细胞的特征与NK细胞相似，（1）不贴壁于培养皿和尼龙棉，（2）表达无唾液酸GM1抗原，（3）缺乏易于检测到的Thy 1抗原和免疫球蛋白。此外，静脉注射无唾液酸GM1完全消除了裸鼠脾细胞中的NK活性，并且在注射抗无唾液酸GM1的小鼠脾细胞中，CGRP引起的脾细胞内cAMP增加较少。我们目前的研究表明，CGRP通过增加细胞内cAMP浓度来抑制NK细胞活性。CGRP可能参与NK功能的调节。
