Yin Yan-Mei, Lu Yu, Zhang Li-Xin, Zhang Guang-Ping, Zhang Zhi-Qiang
Department of Rehabilitation, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.
Synapse. 2015 Mar;69(3):139-47. doi: 10.1002/syn.21802. Epub 2015 Jan 10.
This study investigated the effects of bone marrow stromal cells (BMSCs) transplantation combined with low dose ultrashortwave (USW) radiation on functional recovery from spinal cord injury (SCI) in rats. Female Sprague-Dawley rats (n = 64) were randomly divided into five groups: sham-operated, as well as control, USW, BMSCs, and BMSCs + USW that were subjected to spinal cord contusion. BMSCs were transplanted into rats in the BMSCs and BMSCs+USW groups, while those in the USW and BMSCs + USW were exposed to radiation; Basso-Beattie-Bresnahan (BBB) tests were performed before the operation and at 1-week intervals after SCI for 7 weeks. 7 weeks later, animals were assessed footprint pattern, motor evoked potential (MEP), histological features, and expression of the ED1 and glial fibrillary acidic protein (GFAP), markers of activated macrophages and astrocytes, respectively, in the lesion area. BBB scores were higher in the BMSCs + USW than in the BMSCs and control groups. The USW and BMSCs + USW groups had improved coordination compared to the BMSCs and control groups (P < 0.01 and P < 0.001, respectively, vs. control). BMSCs + USW rats had a lower ratio of lesion/cavity to spinal cord area than the other injured rats, and also had expression of GFAP in transplanted BMSCs. All treatment groups had lower ED1 expression and MEP latency than the controls (P < 0.01 and P < 0.001, respectively). The results indicate that BMSCs transplantation combined with USW radiation is more effective than either treatment alone in promoting functional recovery after SCI, possibly due to concomitant suppression of inflammation and spinal cord repair.
本研究调查了骨髓基质细胞（BMSCs）移植联合低剂量超短波（USW）辐射对大鼠脊髓损伤（SCI）后功能恢复的影响。将64只雌性Sprague-Dawley大鼠随机分为五组：假手术组以及脊髓挫伤后的对照组、超短波组、骨髓基质细胞组和骨髓基质细胞+超短波组。骨髓基质细胞组和骨髓基质细胞+超短波组的大鼠接受骨髓基质细胞移植，而超短波组和骨髓基质细胞+超短波组的大鼠接受辐射；在手术前以及脊髓损伤后每隔1周进行7周的Basso-Beattie-Bresnahan（BBB）测试。7周后，分别评估动物的足迹模式、运动诱发电位（MEP）、组织学特征以及损伤区域中活化巨噬细胞和星形胶质细胞的标志物ED1和胶质纤维酸性蛋白（GFAP）的表达。骨髓基质细胞+超短波组的BBB评分高于骨髓基质细胞组和对照组。与骨髓基质细胞组和对照组相比，超短波组和骨髓基质细胞+超短波组的协调性有所改善（分别与对照组相比，P<0.01和P<0.001）。骨髓基质细胞+超短波组大鼠的损伤/空洞与脊髓面积的比值低于其他损伤大鼠，并且移植的骨髓基质细胞中有GFAP表达。所有治疗组的ED1表达和MEP潜伏期均低于对照组（分别为P<0.01和P<0.001）。结果表明，骨髓基质细胞移植联合超短波辐射在促进脊髓损伤后功能恢复方面比单独任何一种治疗都更有效，这可能是由于同时抑制了炎症和脊髓修复。
