通过白蛋白冠层增强基于纳米颗粒的药物递送系统的生物稳定性。
Enhanced biostability of nanoparticle-based drug delivery systems by albumin corona.
作者信息
Peng Qiang, Wei Xue-Qin, Yang Qin, Zhang Shu, Zhang Ting, Shao Xiao-Ru, Cai Xiao-Xiao, Zhang Zhi-Rong, Lin Yun-Feng
机构信息
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Nanomedicine (Lond). 2015 Jan;10(2):205-14. doi: 10.2217/nnm.14.86.
AIMS
The long-term efficacy of nanoparticles is limited by their rapid metabolism in tissues. In this work, we aim to enhance nanoparticle biostability by preforming a bovine serum albumin (BSA) corona.
MATERIALS & METHODS: A BSA corona was formed by incubating poly-3-hydroxybutyrate-co-3-hydroxyhexanoate nanoparticles with BSA solution and confirmed by SDS-PAGE and x-ray photoelectron spectroscopy. The impacts of the BSA corona on the drug release, biostability and biodistribution of nanoparticles were investigated.
RESULTS
In the presence of the BSA corona, the drug release (coumarin-6 was used as the model drug) of nanoparticles was significantly slower and their stability in liver homogenate and in organs was enhanced.
CONCLUSION
Preformation of a BSA corona may be a promising approach for enhancing drug biostability and for developing long-acting nanoparticle formulations.
目的
纳米颗粒的长期疗效受到其在组织中快速代谢的限制。在本研究中，我们旨在通过形成牛血清白蛋白（BSA）冠层来提高纳米颗粒的生物稳定性。
材料与方法
通过将聚-3-羟基丁酸酯-共-3-羟基己酸酯纳米颗粒与BSA溶液孵育形成BSA冠层，并通过SDS-PAGE和X射线光电子能谱进行确认。研究了BSA冠层对纳米颗粒药物释放、生物稳定性和生物分布的影响。
结果
在存在BSA冠层的情况下，纳米颗粒的药物释放（以香豆素-6作为模型药物）显著减慢，并且它们在肝脏匀浆和器官中的稳定性增强。
结论
形成BSA冠层可能是提高药物生物稳定性和开发长效纳米颗粒制剂的一种有前景的方法。
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