Sousa Mário, Oliveira Elsa, Alves Ângela, Gouveia Mónica, Figueiredo Helena, Ferraz Luís, Barros Alberto, Sá Rosália
Department of Microscopy,Laboratory of Cell Biology (Director),Institute of Biomedical Sciences Abel Salazar (ICBAS),University of Porto,Rua Jorge Viterbo Ferreira,228,4050-313 Porto,Portugal.
Department of Microscopy,Laboratory of Cell Biology,Muldisciplinary Unit for Biomedical Research-UMIB,Institute of Biomedical Sciences Abel Salazar (ICBAS),University of Porto,Rua Jorge Viterbo Ferreira,228,4050-313 Porto,Portugal.
Zygote. 2015 Dec;23(6):900-7. doi: 10.1017/S0967199414000616. Epub 2015 Jan 20.
Asthenozoospermia has been related to structural defects of the sperm flagellum. However, few reports have studied in detail the ultrastructure of sperm with total immotility. We present an ultrastructural study of sperm from five patients with total sperm immotility, four due to dysplasia of the fibrous sheath (DFS) and one with situs-inversus. Of the four patients with DFS, three cases presented a hypertrophic and hyperplastic fibrous sheath that invaded the midpiece space, absence of the annulus, and a short midpiece containing a few disorganized and pale mitochondria. Of these cases, two presented absence of the central complex and radial spokes; another additionally presented absence of dynein arms and nexin bridges; and the other patient presented an intact annulus with a dysplastic fibrous sheath restricted to the principal piece with disorganized microtubule doublets. The patient with situs-inversus presented severe respiratory symptoms, with absence of dynein arms and nexin bridges. In conclusion, we present three cases with DFS associated with total sperm immotility, abnormal mitochondria, and absence of the annulus, central pair complex and radial spokes, of which one had in addition absence of dynein arms and nexin bridges. We also describe a patient, with total sperm immotility and a different presentation of DFS, as the annulus was present and the dysplastic fibrous sheath was restricted to the principal piece. These findings thus confirm the heterogeneity of the DFS condition. The changes observed in the patient with situs-inversus also further support previous observations.
弱精子症与精子鞭毛的结构缺陷有关。然而，很少有报告详细研究完全不动精子的超微结构。我们对五名完全精子不动患者的精子进行了超微结构研究，其中四名患者因纤维鞘发育异常（DFS），一名患者患有内脏反位。在四名DFS患者中，三例表现为纤维鞘肥大和增生，侵入了中段间隙，无环，中段短，含有一些排列紊乱且苍白的线粒体。在这些病例中，两例无中央复合体和辐条；另一例还无动力蛋白臂和连接蛋白桥；另一名患者有完整的环，发育异常的纤维鞘局限于主段，微管双联体排列紊乱。内脏反位患者出现严重的呼吸道症状，无动力蛋白臂和连接蛋白桥。总之，我们报告了三例与完全精子不动、线粒体异常以及无环、中央对复合体和辐条相关的DFS病例，其中一例还无动力蛋白臂和连接蛋白桥。我们还描述了一名完全精子不动且DFS表现不同的患者，因为存在环，发育异常的纤维鞘局限于主段。因此，这些发现证实了DFS情况的异质性。在内脏反位患者中观察到的变化也进一步支持了先前的观察结果。
