Bautch V L
Department of Biology, University of N. Carolina, Chapel Hill 27599.
Mol Biol Med. 1989 Aug;6(4):309-17.
The effects of polyoma (Py) oncogenes in transgenic mice are reviewed, with emphasis on an analysis of mice carrying individual Py early region genes linked to either the Py early region promoter or to the rat insulin II promoter. The perturbations documented in this series of animals varied with both the Py gene and the promoter. Py promoter-driven transgenes led to the development of pituitary tumors and vascular tumors in a gene-dependent manner, suggesting that the Py T antigens have different tissue specificities of transforming activity. The only pathology found in insulin promoter-driven transgenic mice was a beta cell tumor seen in insulin-promoted Py large T antigen-carrying mice. These results also suggest that the Py oncogenes have different tropisms relative to a specific cell type. Analysis of the Py oncogene-induced perturbations may provide both insights into the molecular events controlling tumorigenesis and access to rare or dispersed cell types for further analysis.
本文综述了多瘤病毒（Py）癌基因在转基因小鼠中的作用，重点分析了携带与Py早期区域启动子或大鼠胰岛素II启动子相连的单个Py早期区域基因的小鼠。这一系列动物中记录的扰动因Py基因和启动子而异。Py启动子驱动的转基因以基因依赖的方式导致垂体肿瘤和血管肿瘤的发生，表明Py T抗原具有不同的转化活性组织特异性。在胰岛素启动子驱动的转基因小鼠中发现的唯一病理变化是在携带胰岛素启动子驱动的Py大T抗原的小鼠中出现的β细胞肿瘤。这些结果还表明，Py癌基因相对于特定细胞类型具有不同的嗜性。对Py癌基因诱导的扰动进行分析，可能有助于深入了解控制肿瘤发生的分子事件，并为进一步分析提供获取罕见或分散细胞类型的途径。
