应用多位点可变数目串联重复序列分析在中国鉴定与疫情相关的脑膜炎奈瑟菌C群序列型4821
Application of multiple-locus variable number tandem repeat analysis to identify outbreak-associated Neisseria meningitides serogroup C sequence type 4821 in China.
作者信息
Shan Xiaoying, Zhou Haijian, Zhang Ji, Zhu Bingqing, Xu Li, Hu Guangchun, Bai Aiying, Shao Zhujun, Jiang Baofa
机构信息
Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan City, Shandong Province, P.R. China; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, P.R. China; Jinan Municipal Center for Disease Control and Prevention, Jinan City, Shandong Province, P.R. China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou City, Zhejiang Province, P.R. China.
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, P.R. China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou City, Zhejiang Province, P.R. China.
出版信息
PLoS One. 2015 Jan 20;10(1):e0116422. doi: 10.1371/journal.pone.0116422. eCollection 2015.
Neisseria meningitidis (N. meningitidis) serogroup C sequence type (ST)-4821 caused an outbreak in 2010 in Shandong province of China. Twenty-one non-outbreak-associated strains were isolated, along with twenty-eight N. meningitides serogroup C ST-4821 isolates. Therefore, it's essential to identify and clarify characterization of the real outbreak-associated strains with a rapid method during an outbreak investigation. In this study, multiple-locus variable number tandem repeat analysis (MLVA) was applied to analyze 84 N. meningitidis strains, among which 58 were recovered from two outbreaks and 26 were sporadic isolates. Three MLVA schemes with different combination of VNTR loci were tested, and two of them were suitable for isolates from China: scheme 2 with six loci was found to separate ST into finer resolution, and scheme 3 with five loci can be used to identify outbreak-associated isolates from the same outbreak that caused by N. meningitidis serogroup C ST-4821.
脑膜炎奈瑟菌C群序列型（ST）-4821于2010年在中国山东省引发了一场疫情。除了28株脑膜炎奈瑟菌C群ST-4821分离株外，还分离出了21株非疫情相关菌株。因此，在疫情调查期间，采用快速方法鉴定和阐明真正的疫情相关菌株的特征至关重要。在本研究中，多位点可变数目串联重复序列分析（MLVA）被用于分析84株脑膜炎奈瑟菌菌株，其中58株从两次疫情中分离得到，26株为散发病例分离株。测试了三种具有不同VNTR位点组合的MLVA方案，其中两种适用于来自中国的分离株：发现具有六个位点的方案2能将序列型分辨率提高，具有五个位点的方案3可用于鉴定由脑膜炎奈瑟菌C群ST-4821引起的同一疫情中的疫情相关分离株。
Zotero 插件