对近期出现的与侵袭性脑膜炎球菌病相关的加拿大血清群 W 奈瑟菌 11 型克隆复合体分离株的全基因组分型。

Whole genome typing of the recently emerged Canadian serogroup W Neisseria meningitidis sequence type 11 clonal complex isolates associated with invasive meningococcal disease.

机构信息

Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.

出版信息

Int J Infect Dis. 2018 Apr;69:55-62. doi: 10.1016/j.ijid.2018.01.019. Epub 2018 Feb 4.

DOI:10.1016/j.ijid.2018.01.019

PMID:29414677

Abstract

OBJECTIVES

This study was performed to analyze the Canadian invasive serogroup W Neisseria meningitidis (MenW) sequence type 11 (ST-11) clonal complex (CC) isolates by whole genome typing and to compare Canadian isolates with similar isolates from elsewhere.

METHODS

Whole genome typing of 30 MenW ST-11 CC, 20 meningococcal group C (MenC) ST-11 CC, and 31 MenW ST-22 CC isolates was performed on the Bacterial Isolate Genome Sequence database platform. Canadian MenW ST-11 CC isolates were compared with the 2000 MenW Hajj outbreak strain, as well as with MenW ST-11 CC from other countries.

RESULTS

Whole genome typing showed that the Canadian MenW ST-11 CC isolates were distinct from the traditional MenW ST-22 CC; they were not capsule-switched contemporary MenC strains that incorporated MenW capsules. While some recent MenW disease cases in Canada were caused by MenW ST-11 CC isolates showing relatedness to the 2000 MenW Hajj strain, many were non-Hajj isolates similar to current MenW ST-11 isolates found globally. Geographical and temporal variations in genotypes and surface protein antigen genes were found among the MenW ST-11 CC isolates.

CONCLUSIONS

The current MenW ST-11 isolates did not arise by capsule switching from contemporary MenC ST-11 isolates. Both the Hajj-related and non-Hajj MenW ST-11 CC strains were associated with invasive meningococcal disease in Canada.

摘要

目的

本研究通过全基因组分型分析加拿大侵袭性血清群 W 脑膜炎奈瑟菌（MenW）序列型 11（ST-11）克隆复合体（CC）分离株，并将加拿大分离株与其他地方的类似分离株进行比较。

方法

在细菌分离基因组序列数据库平台上对 30 株 MenW ST-11 CC、20 株脑膜炎奈瑟菌 C 组（MenC）ST-11 CC 和 31 株 MenW ST-22 CC 分离株进行全基因组分型。将加拿大 MenW ST-11 CC 分离株与 2000 年 Hajj 流行株以及其他国家的 MenW ST-11 CC 进行比较。

结果

全基因组分型显示，加拿大 MenW ST-11 CC 分离株与传统的 MenW ST-22 CC 不同；它们不是包含 MenW 荚膜的当代 MenC 菌株的荚膜转换。虽然加拿大最近的一些 MenW 疾病病例是由与 2000 年 Hajj 株具有亲缘关系的 MenW ST-11 CC 分离株引起的，但许多非 Hajj 分离株与全球目前发现的 MenW ST-11 分离株相似。MenW ST-11 CC 分离株的基因型和表面蛋白抗原基因存在地理和时间变异。