Calder Bridget, Soares Nelson C, de Kock Elise, Blackburn Jonathan M
Division of Medical Biochemistry, Faculty of Health Sciences, Institute of Infectious Diseases and Molecular Medicine (IDM), University of Cape Town, Anzio Rd, Observatory, Cape Town 7925, South Africa.
Expert Rev Proteomics. 2015 Feb;12(1):21-35. doi: 10.1586/14789450.2015.1007046.
The Mycobacterium tuberculosis bacillus has a number of unique features that make it a particularly effective human pathogen. Although genomic analysis has added to our current understanding of the molecular basis by which M. tuberculosis damages its host, proteomics may be better suited to describe the dynamic interactions between mycobacterial and host systems that underpin this disease. The M. tuberculosis proteome has been investigated using proteomics for over a decade, with increasingly sophisticated mass spectrometry technology and sensitive methods for comparative proteomic profiling. Deeper coverage of the M. tuberculosis proteome has led to the identification of hundreds of putative virulence determinants, as well as an unsurpassed coverage of post-translational modifications. Proteomics is therefore uniquely poised to contribute to our understanding of this pathogen, which may ultimately lead to better management of the disease.
结核分枝杆菌具有许多独特特征，使其成为一种特别有效的人类病原体。尽管基因组分析增进了我们目前对结核分枝杆菌损害宿主的分子基础的理解，但蛋白质组学可能更适合描述支撑这种疾病的分枝杆菌与宿主系统之间的动态相互作用。十多年来，一直使用蛋白质组学对结核分枝杆菌蛋白质组进行研究，采用了日益复杂的质谱技术和用于比较蛋白质组分析的灵敏方法。对结核分枝杆菌蛋白质组更深入的覆盖已导致鉴定出数百种假定的毒力决定因素，以及对翻译后修饰的无与伦比的覆盖。因此，蛋白质组学在促进我们对这种病原体的理解方面具有独特的优势，这最终可能导致对该疾病的更好管理。
