Singh K M, Pandya P R, Tripathi A K, Patel G R, Parnerkar S, Kothari R K, Joshi C G
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, Gujarat, India.
Animal Nutrition Research Station, AAU, Anand, Gujarat, India.
Meta Gene. 2014 Feb 19;2:191-9. doi: 10.1016/j.mgene.2014.01.001. eCollection 2014 Dec.
The aim of this study was to detect the major bacteria present in rumen microbiota. Here, we performed qPCR based absolute quantitation of selected rumen microbes in rumen fluid of river buffalo adapted to varying proportion of concentrate to roughage diets. Animals were adapted to roughage-to-concentrate ratio in the proportion of 100:00 (T1), 75:25 (T2), 50:50 (T3) and 25:75 (T4) respectively for 30 days. At the end of each treatment, rumen fluid was collected at 0 h and 2 h after feeding. It was found that among fibrolytic bacteria Ruminococcus flavefaciens (2.22 × 10(8) copies/ml) were highest in T2 group and followed by 1.11 × 10(8) copies/ml for Fibrobacter succinogenes (T2), 2.56 × 10(7) copies/ml for Prevotella ruminicola (T1) and 1.25 × 10(7) copies/ml for Ruminococcus albus (T4). In non-fibrolytic bacteria, the Selenomonas ruminantium (2.62 × 10(7) copies/ml) was predominant in group T3 and followed by Treponema bryantii (2.52 × 10(7)copies/ml) in group T1, Ruminobacter amylophilus (1.31 × 10(7)copies/ml) in group T1 and Anaerovibrio lipolytica (2.58 × 10(6) copies/ml) in group T4. It is most notable that R. flavefaciens were the highest in population in the rumen of Surti buffalo fed wheat straw as roughage source.
本研究的目的是检测瘤胃微生物群中存在的主要细菌。在此，我们对适应不同精粗比日粮的河水牛瘤胃液中选定的瘤胃微生物进行了基于qPCR的绝对定量分析。动物分别以100:00（T1）、75:25（T2）、50:50（T3）和25:75（T4）的粗精比适应30天。在每种处理结束时，于采食后0小时和2小时采集瘤胃液。结果发现，在纤维分解菌中，黄化瘤胃球菌（2.22×10⁸拷贝/ml）在T2组中含量最高，其次是琥珀酸纤维杆菌（T2组，1.11×10⁸拷贝/ml）、瘤胃普雷沃氏菌（T1组，2.56×10⁷拷贝/ml）和白色瘤胃球菌（T4组，1.25×10⁷拷贝/ml）。在非纤维分解菌中，反刍月形单胞菌（2.62×10⁷拷贝/ml）在T3组中占主导地位，其次是T1组中的布氏密螺旋体（2.52×10⁷拷贝/ml）、T1组中的嗜淀粉瘤胃杆菌（1.31×10⁷拷贝/ml）和T4组中的解脂厌氧弧菌（2.58×10⁶拷贝/ml）。最值得注意的是，以小麦秸秆为粗饲料来源的苏提水牛瘤胃中，黄化瘤胃球菌的数量最多。
