利用主成分分析和层次聚类剖析印度东北部姜花属植物的遗传多样性及亲缘关系。

Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering.

作者信息

Basak Supriyo, Ramesh Aadi Moolam, Kesari Vigya, Parida Ajay, Mitra Sudip, Rangan Latha

机构信息

Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam 781 039, India.

M S Swaminathan Research Foundation, 3rd Cross Street, Taramani Institutional area, Chennai 600 113, India.

出版信息

Meta Gene. 2014 Jul 1;2:459-68. doi: 10.1016/j.mgene.2014.05.002. eCollection 2014 Dec.

DOI:10.1016/j.mgene.2014.05.002

PMID:25606430

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4287868/

Abstract

Molecular genetic fingerprints of eleven Hedychium species from Northeast India were developed using PCR based markers. Fifteen inter-simple sequence repeats (ISSRs) and five amplified fragment length polymorphism (AFLP) primers produced 547 polymorphic fragments. Positive correlation (r = 0.46) was observed between the mean genetic similarity and genetic diversity parameters at the inter-species level. AFLP and ISSR markers were able to group the species according to its altitude and intensity of flower aroma. Cophenetic correlation coefficients between the dendrogram and the original similarity matrix were significant for ISSR (r = 0.89) compared to AFLP (r = 0.83) markers. This genetic characterization of Hedychium from Northeast India contributes to the knowledge of genetic structure of the species and can be used to define strategies for their conservation and management.

摘要

利用基于聚合酶链反应（PCR）的标记技术，构建了来自印度东北部的11种姜花属植物的分子遗传指纹图谱。15个简单重复序列区间（ISSR）引物和5个扩增片段长度多态性（AFLP）引物共产生了547个多态性片段。在种间水平上，平均遗传相似性与遗传多样性参数之间呈正相关（r = 0.46）。AFLP和ISSR标记能够根据物种的海拔高度和花香强度对其进行分组。与AFLP标记（r = 0.83）相比，ISSR标记（r = 0.89）的树状图与原始相似性矩阵之间的协同相关系数更为显著。对印度东北部姜花属植物的这种遗传特征分析有助于了解该物种的遗传结构，并可用于制定其保护和管理策略。