Centre of Excellence in Biotechnology, M.P. Council of Science and Technology, Vigyan Bhawan, Nehru Nagar, Bhopal, Madhya Pradesh, 462003, India.
Mol Biol Rep. 2021 Mar;48(3):2437-2452. doi: 10.1007/s11033-021-06278-0. Epub 2021 Mar 25.
Gloriosa superba L., an endangered medicinal plant with global interest due to presence of colchicine, an important alkaloid used in formulations of Indian and Traditional medicine. The plant has become endangered due to its unscientifically exploitation and high medicinal values. In the Present study 10 randomly amplified polymorphic DNA (RAPD) and 6 ISSR markers were employed to assess genetic divergence among micro propagated, wild and field cultivated plants of Gloriosa superba collected from different parts of India. In RAPD analysis, all the 10 accession with 10 RAPD primers amplified 466 fragments, with 96.43 % polymorphism and with an average of 46.6 bands per primer. The size of amplicons varied from 1656 to 100 bp. While, ISSR primers produced 328 fragments of which 298 were polymorphic with an average of 49.7 bands per primer with 91.83% polymorphism. The size of amplicons ranges from 2395 to 181 bp. RAPD, ISSR markers were also assessed by calculating polymorphic information content (PIC) to discriminate the genotypes, Average PIC value for RAPD, ISSR and combined RAPD + ISSR markers obtained was ≤ 0.50 suggesting the informativeness of markers. Jaccard's coefficient ranges from 0.18 to 0.75 (RAPD) and 0.17 to 0.61 (ISSR) and 0.21-0.52 for pooled ISSR and RAPD markers. The clustering pattern based on UPGMA analysis of the genotypes in the combined analysis revealed that the majority of the genotypes remained similar to the ISSR dendrogram, while the RAPD-based dendrogram showed some variation in the clustering of genotypes. The result of PCA scattered plot obtained were in agreement with the UPGMA dendrogram, which further confirms the genetic relationships explain by cluster analysis. Results confirmed that the genotype studied had good genetic diversity and can be used for identification, conservation, and future breeding program of Gloriosa species and consequently for the benefit of the pharmaceutical industries.
宝盖草 L.，一种濒危药用植物，因其含有秋水仙碱而备受全球关注，秋水仙碱是一种重要的生物碱，用于印度和传统医学的制剂。由于其不合理的开发和高药用价值，该植物已濒临灭绝。在本研究中，采用 10 个随机扩增多态性 DNA（RAPD）和 6 个 ISSR 标记评估了来自印度不同地区的微繁殖、野生和田间栽培的宝盖草的遗传分化。在 RAPD 分析中，所有 10 个访问号用 10 个 RAPD 引物扩增了 466 个片段，多态性为 96.43%，每个引物平均有 46.6 个带。扩增子的大小从 1656 到 100bp 不等。而 ISSR 引物产生了 328 个片段，其中 298 个具有多态性，每个引物的平均多态性带数为 49.7，多态性为 91.83%。扩增子的大小范围从 2395 到 181bp。RAPD、ISSR 标记也通过计算多态信息含量（PIC）来区分基因型，RAPD、ISSR 和组合 RAPD+ISSR 标记的平均 PIC 值均≤0.50，表明标记的信息量。Jaccard 系数范围为 0.18-0.75（RAPD）和 0.17-0.61（ISSR），组合的 ISSR 和 RAPD 标记的系数范围为 0.21-0.52。基于组合分析中基因型的 UPGMA 分析的聚类模式表明，大多数基因型与 ISSR 树状图相似，而基于 RAPD 的树状图显示基因型聚类存在一些变化。PCA 散点图的结果与 UPGMA 树状图一致，进一步证实了聚类分析解释的遗传关系。结果证实，所研究的基因型具有良好的遗传多样性，可用于宝盖草属的鉴定、保护和未来的育种计划，从而造福制药工业。
