let-65是秀丽隐杆线虫中的细胞质甲硫氨酰tRNA合成酶。

let-65 is cytoplasmic methionyl tRNA synthetase in C. elegans.

作者信息

Alriyami Maha Z, Jones Martin R, Johnsen Robert C, Banerjee Yajnavalka, Baillie David L

机构信息

Department of Molecular Biology and Biochemistry, Simon Fraser University Burnaby, 8888 University Drive, Burnaby, BC V5A 1S6 Canada ; Department of Biochemistry, College of Medicine and Health Sciences, Sultan Qaboos University, 35, Al-Khod 123, Oman.

Genome Sciences Centre, British Columbia Cancer Research Centre, 600 West 10th Avenue, Vancouver, BC V5Z 4E6, Canada.

出版信息

Meta Gene. 2014 Nov 9;2:819-30. doi: 10.1016/j.mgene.2014.08.006. eCollection 2014 Dec.

DOI:10.1016/j.mgene.2014.08.006

PMID:25606464

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4287814/

Abstract

Cytoplasmic methionyl tRNA synthetase (MetRS) is one of more than 20 cytoplasmic aminoacyl tRNA synthetase enzymes (ARS). This family of enzymes catalyzes a process fundamental for protein translation. Using a combination of genetic mapping, oligonucleotide array comparative genomic hybridization, and phenotypic correlation, we show that mutations in the essential gene, let-65, reside within the predicted Caenorhabditis elegans homologue of MetRS, which we have named mars-1. We demonstrate that the lethality associated with alleles of let-65 is fully rescued by a transgenic array that spans the mars-1 genomic region. Furthermore, sequence analysis reveals that six let-65 alleles lead to the alteration of highly conserved amino acids.

摘要

细胞质甲硫氨酰tRNA合成酶（MetRS）是20多种细胞质氨酰tRNA合成酶（ARS）之一。该酶家族催化蛋白质翻译的一个基本过程。通过基因定位、寡核苷酸阵列比较基因组杂交和表型相关性相结合的方法，我们发现必需基因let-65中的突变位于预测的秀丽隐杆线虫MetRS同源物内，我们将其命名为mars-1。我们证明，与let-65等位基因相关的致死性可被跨越mars-1基因组区域的转基因阵列完全挽救。此外，序列分析表明，六个let-65等位基因导致高度保守氨基酸的改变。