Abdullah Azila, Olsen Christel M, Hodneland Kjartan, Rimstad Espen
Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, P.O. Box 8146 Dep, 0033 Oslo, Norway.
MSD Animal Health Norway, Thormøhlensgate 55, N-5008 Bergen, Norway.
Viruses. 2015 Jan 19;7(1):252-67. doi: 10.3390/v7010252.
Vaccination is an important strategy for the control and prevention of infectious pancreatic necrosis (IPN) in farmed Atlantic salmon (Salmo salar) in the post-smolt stage in sea-water. In this study, a heterologous gene expression system, based on a replicon construct of salmonid alphavirus (SAV), was used for in vitro and in vivo expression of IPN virus proteins. The large open reading frame of segment A, encoding the polyprotein NH2-pVP2-VP4-VP3-COOH, as well as pVP2, were cloned and expressed by the SAV replicon in Chinook salmon embryo cells (CHSE-214) and epithelioma papulosum cyprini (EPC) cells. The replicon constructs pSAV/polyprotein (pSAV/PP) and pSAV/pVP2 were used to immunize Atlantic salmon (Salmo salar) by a single intramuscular injection and tested in a subsequent IPN virus (IPNV) challenge trial. A low to moderate protection against IPN was observed in fish immunized with the replicon vaccine that encoded the pSAV/PP, while the pSAV/pVP2 construct was not found to induce protection.
疫苗接种是海水养殖大西洋鲑（Salmo salar）后幼鲑阶段控制和预防传染性胰腺坏死（IPN）的重要策略。在本研究中，基于鲑鱼α病毒（SAV）复制子构建体的异源基因表达系统被用于IPN病毒蛋白的体外和体内表达。编码多聚蛋白NH2-pVP2-VP4-VP3-COOH的A节段的大开放阅读框以及pVP2，通过SAV复制子在奇努克鲑胚胎细胞（CHSE-214）和鲤上皮瘤（EPC）细胞中进行克隆和表达。复制子构建体pSAV/多聚蛋白（pSAV/PP）和pSAV/pVP2通过单次肌肉注射用于免疫大西洋鲑（Salmo salar），并在随后的IPN病毒（IPNV）攻毒试验中进行测试。在用编码pSAV/PP的复制子疫苗免疫的鱼中观察到对IPN的低至中度保护，而未发现pSAV/pVP2构建体诱导保护作用。
