Paludan K, Duch M, Jørgensen P, Kjeldgaard N O, Pedersen F S
Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark.
Gene. 1989 Dec 28;85(2):421-6. doi: 10.1016/0378-1119(89)90435-6.
The effects of Geneticin (G418) selection on the growth and survival of cultured mammalian cells expressing the neomycin-resistance gene (neo) were studied by the analysis of cell clones from two retroviral neo vector-infected populations. We found a correlation between the neo expression level and growth rates in medium containing varying G418 concentrations. This relationship permits the use of differential selection schemes for the isolation of rare cells with increased expression. Comparison, by clone sampling, of vector-positive populations before and after selection with a G418 concentration in the range usually used for selection, showed different expression level and vector copy number distributions for the population infected with the vector of lower LTR activity, but not for the other. Such biasing effects of G418 selection may be important when selected cells are used for quantitative studies of gene expression.
通过对两个逆转录病毒新霉素抗性基因（neo）载体感染群体的细胞克隆进行分析，研究了遗传霉素（G418）选择对表达新霉素抗性基因的培养哺乳动物细胞生长和存活的影响。我们发现新霉素表达水平与含有不同G418浓度的培养基中的生长速率之间存在相关性。这种关系允许使用差异选择方案来分离表达增加的稀有细胞。通过克隆取样比较在通常用于选择的G418浓度下选择前后的载体阳性群体，发现感染低LTR活性载体的群体具有不同的表达水平和载体拷贝数分布，而另一个群体则没有。当选择的细胞用于基因表达的定量研究时，G418选择的这种偏差效应可能很重要。
