Tabucchi A, Terzuoli L, Di Stefano A, Pizzichini M, Leoncini R, Dispensa E, Marinello E
Boll Soc Ital Biol Sper. 1989 Nov;65(11):1097-103.
Phosphoribosyl-1-pyrophosphate (PRPP) amidotransferase is the "key anabolic enzyme" of purine nucleotide synthesis; PRPP synthetase connects the pentose cycle with the same pathway. We have studied their behavior in 5 control subjects and in 8 affected by CLL. Determination of PRPP amidotransferase was carried out through the evaluation of 14C-glutamic acid (released by 14C-glutamine) in the incubation mixture. PRPP synthetase was followed by adding ATP and ribose 5-phosphate to the incubation mixtures, and by evaluating the PRPP formed through the release of CO2 in a coupled reaction. In the case of PRPP-amidotransferase, our values are in the range reported in the literature: in patients affected by CLL, the enzyme activity is much higher and the increase is more evident when values referred to the patients, than when to the cells. Our values of PRPP synthetase are consistent with those of Peters and Veerkamp, but no definite conclusion is possible in the case of leukemic patients.
磷酸核糖-1-焦磷酸（PRPP）酰胺转移酶是嘌呤核苷酸合成的“关键合成酶”；PRPP合成酶将戊糖循环与同一途径相连。我们研究了5名对照受试者和8名慢性淋巴细胞白血病（CLL）患者中它们的行为。通过评估孵育混合物中14C-谷氨酸（由[sup]14[/sup]C-谷氨酰胺释放）来测定PRPP酰胺转移酶。通过向孵育混合物中添加ATP和5-磷酸核糖，并通过评估在偶联反应中通过释放CO[sub]2[/sub]形成的PRPP来跟踪PRPP合成酶。就PRPP酰胺转移酶而言，我们的值在文献报道的范围内：在CLL患者中，该酶活性高得多，与患者相关的值相比，与细胞相关的值时增加更明显。我们的PRPP合成酶值与彼得斯和费坎普的值一致，但对于白血病患者无法得出明确结论。
