Benitez-Herreros Javier, Lopez-Guajardo Lorenzo, Vazquez-Blanco Miguel, Perez-Crespo Aurora, Silva-Mato Agustin
a Department of Ophthalmology , University Hospital Principe de Asturias , Alcalá de Henares, Madrid , Spain .
b Department of Surgery, Medical and Social Sciences, Faculty of Medicine , Alcalá University , Madrid , Spain and.
Curr Eye Res. 2016;41(1):129-32. doi: 10.3109/02713683.2014.1002050. Epub 2015 Jan 22.
To assess the influence that hydration applied on the sclerotomy edges may have on incisional closure resistance after transconjunctival sutureless vitrectomy (TSV).
Experimental, randomized and observer-masked study in which 23-gauge TSV was performed in 80 cadaveric pig eyes. Once each vitrectomy was finished, hydration with balanced salt solution (BSS) was applied on the sclerotomy edges of one of the superior incision sites; no maneuver was performed on the other superior sclerotomy. Intraocular pressure (IOP) was gradually increased by means of the vitrectomy system (Accurus; Alcon Laboratories, TX) until one of the superior sclerotomies opened, allowing internal ocular solution to escape.
In 45% of cases (36 of 80 eyes), sclerotomies subjected to hydration allowed intraocular fluid escape (p = 0.43). There were no differences when comparing opening pressure values of hydrated and non-hydrated sclerotomies (p = 0.19).
Scleral hydration did not demonstrate increase in the sclerotomy closure resistance in our experimental model. Given the widespread use of sutureless TSV around the world, the results obtained in our research, in spite of being negative, may contribute to the knowledge of the behavior of sutureless sclerotomies.
评估经结膜无缝线玻璃体切除术(TSV)后,对巩膜切口边缘进行水合作用可能对切口闭合阻力产生的影响。
进行实验性、随机且观察者盲法研究,对80只猪尸体眼实施23G TSV。每次玻璃体切除完成后,对上方其中一个切口部位的巩膜切口边缘用平衡盐溶液(BSS)进行水合处理;对另一个上方巩膜切口不做处理。通过玻璃体切除系统(Accurus;爱尔康实验室,德克萨斯州)逐渐升高眼内压(IOP),直至上方其中一个巩膜切口裂开,使眼内液体流出。
在45%的病例(80眼中的36眼)中,接受水合处理的巩膜切口出现眼内液体流出(p = 0.43)。比较水合处理和未水合处理的巩膜切口的裂开压力值时,未发现差异(p = 0.19)。
在我们的实验模型中,巩膜水合作用并未显示出巩膜切口闭合阻力增加。鉴于无缝线TSV在全球的广泛应用,尽管我们的研究结果为阴性,但所获结果可能有助于了解无缝线巩膜切口的情况。