Doane Tennyson L, Alam Rabeka, Maye Mathew M
Department of Chemistry, Syracuse University, Syracuse New York, 13244, USA.
Nanoscale. 2015 Feb 21;7(7):2883-8. doi: 10.1039/c4nr07662a.
The DNA-mediated self-assembly of CdSe/CdS quantum rods (QRs) onto DNA origami is described. Two QR types with unique optical emission and high polarization were synthesized, and then functionalized with oligonucleotides (ssDNA) using a novel protection-deprotection approach, which harnessed ssDNA's tailorable rigidity and denaturation temperature to increase DNA coverage by reducing non-specific coordination and wrapping. The QR assembly was programmable, and occurred at two different assembly zones that had capture strands in parallel alignment. QRs with different optical properties were assembled, opening up future studies on orientation dependent QR FRET. The QR-origami conjugates could be purified via gel electrophoresis and sucrose gradient ultracentrifugation. Assembly yields, QR stoichiometry and orientation, as well as energy transfer implications were studied in light of QR distances, origami flexibility, and conditions.
描述了CdSe/CdS量子棒（QRs）在DNA折纸结构上的DNA介导自组装。合成了两种具有独特光发射和高极化特性的QR类型，然后使用一种新颖的保护-去保护方法用寡核苷酸（单链DNA）进行功能化，该方法利用单链DNA可定制的刚性和变性温度，通过减少非特异性配位和包裹来增加DNA覆盖率。QR组装是可编程的，并且发生在两个具有平行排列捕获链的不同组装区域。组装了具有不同光学性质的QR，为未来关于取向依赖性QR荧光共振能量转移（FRET）的研究开辟了道路。QR-折纸缀合物可通过凝胶电泳和蔗糖梯度超速离心进行纯化。根据QR距离、折纸结构的柔韧性和条件，研究了组装产率、QR化学计量和取向以及能量转移的影响。
