Dong Wei, Zhou Siqi, Dong Yan, Wang Jingwen, Ge Xin, Sui Lili
Department of Chemistry, Shenyang Medical College, Shenyang, People's Republic of China.
ICU, Fengtian Hospital Affiliated to Shenyang Medical College, Shenyang, People's Republic of China.
Luminescence. 2015 Sep;30(6):867-71. doi: 10.1002/bio.2834. Epub 2015 Jan 21.
In this work, fluorescent carbon dots (CDs) were synthesized using a hydrothermal method with glucose as the carbon source and were surface-modified with ethylenediamine. The properties of as-prepared CDs were analyzed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR), ultraviolet-visible light (UV/vis) absorption and fluorescent spectra. Furthermore, CDs conjugated with mouse anti-(human carcinoembryonic antigen) (CEA) monoclonal antibody were successful employed in the biolabeling and fluorescent imaging of human gastric carcinoma cells. In addition, the cytotoxicity of CDs was also tested using human gastric carcinoma cells. There was no apparent cytotoxicity on human gastric carcinoma cells. These results suggest the potential application of the as-prepared CDs in bioimaging and related fields.
在本研究中，以葡萄糖为碳源，采用水热法合成了荧光碳点（CDs），并用乙二胺对其进行表面改性。通过透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、紫外-可见光（UV/vis）吸收光谱和荧光光谱对所制备的CDs的性质进行了分析。此外，与小鼠抗（人癌胚抗原）（CEA）单克隆抗体偶联的CDs成功应用于人胃癌细胞的生物标记和荧光成像。此外，还用人胃癌细胞测试了CDs的细胞毒性。对人胃癌细胞没有明显的细胞毒性。这些结果表明所制备的CDs在生物成像及相关领域具有潜在的应用价值。
