Jiang Chao, Hou Jing-Yi, Huang Lu-Qi, Yuan Yuan, Chen Min, Jin Yan
Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(19):3668-72.
To simply and rapid authenticate Lonicera japanica. Rapid allele-specific PCR primer was designed base on trnL-trnF 625 G/T Single nucleotide polymorphism and the PCR reaction systems including annealing temperature was optimized; optimized results were performed to authenticate L. japanica and its 9 adulterants. When 100 x SYBR Green I was added in the PCR product of 87 degrees C initial denatured 1 min; 87 degrees C denatured 5 s, 68 degrees C annealing 5 s, 30 cycle; L. japanica visualize strong green fluorescence under 365 nm UV lamp whereas adulterants without. The results indicate rapid allele-specific PCR could authenticate L. japanica and its adulterants rapidly and simply.
为了简单快速地鉴定忍冬。基于trnL-trnF 625 G/T单核苷酸多态性设计了快速等位基因特异性PCR引物，并对包括退火温度在内的PCR反应体系进行了优化；将优化结果用于鉴定忍冬及其9种掺伪品。当在87℃初始变性1分钟的PCR产物中加入100x SYBR Green I时；87℃变性5秒，68℃退火5秒，30个循环；忍冬在365nm紫外灯下呈现强烈的绿色荧光，而掺伪品则没有。结果表明，快速等位基因特异性PCR能够快速、简单地鉴定忍冬及其掺伪品。
