Laboratorio de Morfometria, Metabolismo e Doença Cardiovascular, Centro Biomedico, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Av 28 de Setembro 87 fds, Rio de Janeiro, RJ, 20551-030, Brazil.
Eur J Nutr. 2016 Feb;55(1):159-69. doi: 10.1007/s00394-015-0834-0. Epub 2015 Jan 23.
Fish oil (FO) elicits diverse beneficial effects. Reduction in or prevention of body mass (BM) gain in animal models may be associated with modulation of brown adipose tissue (BAT). We aimed to evaluate the effects of different high-fat diets with FO on BAT metabolism and thermogenic markers.
C57BL/6 male mice (3-month-old) were fed different diets during 8 weeks: standard-chow diet (SC 10% fat), high-fat lard diet (HF-L 50% fat), high-fat lard plus FO diet (HF-L+FO 50% fat), and high-fat FO diet (HF-FO 50% fat). We evaluated BM and performed an oral glucose tolerance test. At euthanasia, plasma was collected for leptin, and triacylglycerol measurement and interscapular BAT was dissected and stored for molecular analyses.
HF-L group showed elevated BM; glucose intolerance associated with diminished TC10 and GLUT4 expressions; hypertriglyceridemia associated with increased CD36 and diminished CPT1 expression; elevated expression of pro-inflammatory cytokines; and reduced PPAR expression. Furthermore, these animals showed hyperleptinemia with increased expression of thermogenic markers (beta3-AR, PGC1alpha, and UCP1). Conversely, HF-L+FO and HF-FO groups showed reduced BM gain with regularization of glucose tolerance and triglyceridemia, GLUT4, TC10, CD36, CPT1, and cytokines expressions. Both groups exhibited elevated PPAR and thermogenic markers expression in a dose-dependent way.
FO improves metabolic profile and upregulates thermogenic markers, suggesting an elevated thermogenesis that leads to reduced BM gain.
鱼油（FO）具有多种有益作用。动物模型中体重（BM）增加的减少或预防可能与棕色脂肪组织（BAT）的调节有关。我们旨在评估不同高脂肪饮食中添加 FO 对 BAT 代谢和产热标志物的影响。
3 月龄 C57BL/6 雄性小鼠分别喂食不同的饮食 8 周：标准饲料（SC 10%脂肪）、高脂肪猪油饮食（HF-L 50%脂肪）、高脂肪猪油加 FO 饮食（HF-L+FO 50%脂肪）和高脂肪 FO 饮食（HF-FO 50%脂肪）。我们评估了 BM 并进行了口服葡萄糖耐量试验。安乐死后，收集血浆以测量瘦素和三酰甘油，并解剖和储存肩胛间 BAT 以进行分子分析。
HF-L 组表现出 BM 增加；葡萄糖耐量受损与 TC10 和 GLUT4 表达减少有关；高甘油三酯血症与 CD36 增加和 CPT1 表达减少有关；促炎细胞因子表达增加；以及 PPAR 表达减少。此外，这些动物表现出高瘦素血症和产热标志物（β3-AR、PGC1alpha 和 UCP1）表达增加。相反，HF-L+FO 和 HF-FO 组表现出 BM 增加减少，葡萄糖耐量和甘油三酯正常化，GLUT4、TC10、CD36、CPT1 和细胞因子表达正常化。两组均表现出 PPAR 和产热标志物表达的剂量依赖性增加。
FO 改善了代谢谱并上调了产热标志物，表明产热增加导致 BM 增加减少。
