Luan Chunguang, Xie Lingling, Yang Xi, Miao Huifang, Lv Na, Zhang Ruifen, Xiao Xue, Hu Yongfei, Liu Yulan, Wu Na, Zhu Yuanmin, Zhu Baoli
1] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China [2] University of Chinese Academy of Sciences, Beijing 100049, China.
Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China.
Sci Rep. 2015 Jan 23;5:7980. doi: 10.1038/srep07980.
The fungal microbiota is an important component of the human gut microbiome and may be linked to gastrointestinal disease. In this study, the fungal microbiota of biopsy samples from adenomas and adjacent tissues was characterized by deep sequencing. Ascomycota, Glomeromycota and Basidiomycota were identified as the dominant phyla in both adenomas and adjacent tissues from all subjects. Among the 60 genera identified, the opportunist pathogens Phoma and Candida represented an average of 45% of the fungal microbiota. When analyzed at the operational taxonomic unit (OTU) level, however, a decreased diversity in adenomas was observed, and three OTUs differed significantly from the adjacent tissues. Principal Component Analysis (PCA) revealed that the core OTUs formed separate clusters for advanced and non-advanced adenomas for which the abundance of four OTUs differed significantly. Moreover, the size of adenomas and the disease stage were closely related to changes in the fungal microbiota in subjects with adenomas. This study characterized the fungal microbiota profile of subjects with adenomas and identified potential diagnostic biomarkers closely related to different stages of adenomas.
真菌微生物群是人类肠道微生物组的重要组成部分，可能与胃肠道疾病有关。在本研究中，通过深度测序对腺瘤及相邻组织活检样本的真菌微生物群进行了表征。子囊菌门、球囊菌门和担子菌门被确定为所有受试者腺瘤和相邻组织中的优势菌门。在鉴定出的60个属中，机会致病菌茎点霉属和念珠菌属平均占真菌微生物群的45%。然而，在操作分类单元（OTU）水平进行分析时，观察到腺瘤中的多样性降低，并且有三个OTU与相邻组织有显著差异。主成分分析（PCA）显示，核心OTU为进展期和非进展期腺瘤形成了单独的聚类，其中四个OTU的丰度有显著差异。此外，腺瘤的大小和疾病阶段与腺瘤患者真菌微生物群的变化密切相关。本研究表征了腺瘤患者的真菌微生物群特征，并确定了与腺瘤不同阶段密切相关的潜在诊断生物标志物。
