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[Localization in the body of the mobile element MDG1 of 2 regions specifically binding with Drosophila nuclear proteins].

作者信息

Cherkasova V A, Sedkov Iu A, Karavanov A A, Il'in Iu V

机构信息

V. A. Engelgardt Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow.

出版信息

Genetika. 1989 Dec;25(12):2101-10.

PMID:2561358
Abstract

Two regions in mdg1 mobile element's body can specifically bind nuclear proteins of Drosophila melanogaster, as demonstrated by the method of retention of DNA-protein complexes of nitrocellulose filters. The first region is situated in the 5'-end part of mdg1, 1 kb downstream the site of initiation of transcription and contains long oligo (A) blocks (from 14 to 30 nucleotides) in the coding chain. The second region is localized near the 3'-LTR and consists of tandem 14-nucleotide repeats and a palindrome, destruction of which leads to weaker binding. There is no competition between the two regions for proteins, which evidence that they are recognized by the different proteins. The binding with the first region can be suppressed by adding the 412 mobile element DNA. These regions are supposed to take place in the regulation of mdg1 transcription.

摘要

相似文献

1
[Localization in the body of the mobile element MDG1 of 2 regions specifically binding with Drosophila nuclear proteins].
Genetika. 1989 Dec;25(12):2101-10.
2
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3
[Interaction of products of su(Hw) and su(f) genes with MDG4 region regulating its transcription suppresses mutations in Drosophila caused by insertion of this gene].[su(Hw)和su(f)基因产物与调控其转录的MDG4区域的相互作用抑制果蝇中由该基因插入引起的突变]
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引用本文的文献

1
Leader region of mdg1 Drosophila retrotransposon RNA contains 3'-end processing sites.果蝇反转录转座子mdg1 RNA的引导区域包含3'末端加工位点。
Nucleic Acids Res. 1991 Jun 25;19(12):3213-9. doi: 10.1093/nar/19.12.3213.