Fan Cui-Fang, Zhu An-Na, Huang Ting-Ting, Li Lu, Wang Su-Qing
Department of Obstetrics , Renmin Hospital, Wuhan University, Wuhan 430071, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Jan;35(1):72-6.
To investigate the inhibitory effects of tetramethoxystilbene, a selective CYP1B1 inhibitor, on adipogenic differentiation of C3H10T1/2 multi-potent mesenchymal cells.
In vitro cultured C3H10T1/2 cells at full confluence were induced by adipogenic agents (10 µg/ml insulin, 2 µmol/L dexamethasone and 0.5 mmol/L 3-isobutyl-1-methylxanthine) and exposed simultaneously to TMS at the final concentrations of 1.0, 2.0 or 4.0 µg/ml. Oil Red-O staining was used to observe the cell differentiation. The expression of peroxisome proliferator-activated receptor gamma (PPARγ) and its target genes cluster of differentiation 36 (CD36) and fatty acid binding protein 4 (FABP4) were quantified by real-time RT-PCR and Western blotting.
Oil Red-O staining and TG contents revealed that TMS suppressed induced differentiation of C3H10T1/2 cells. TMS exposure of the cells dose-dependently decreased both mRNA and protein expressions of PPARγ, a key nuclear transcription factor during adipogenesis, and also lowered the mRNA expressions of PPARγ target genes CD36 and FABP4.
TMS can suppress adipogenic differentiation of C3H10T1/2 cells by inhibiting PPARγ
研究选择性CYP1B1抑制剂四甲氧基芪对C3H10T1/2多能间充质细胞成脂分化的抑制作用。
将体外培养至完全汇合的C3H10T1/2细胞用成脂诱导剂（10μg/ml胰岛素、2μmol/L地塞米松和0.5mmol/L 3-异丁基-1-甲基黄嘌呤）诱导，并同时分别用终浓度为1.0、2.0或4.0μg/ml的四甲氧基芪处理。采用油红O染色观察细胞分化情况。通过实时逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测过氧化物酶体增殖物激活受体γ（PPARγ）及其靶基因分化抗原36（CD36）和脂肪酸结合蛋白4（FABP4）的表达。
油红O染色及甘油三酯含量检测显示，四甲氧基芪可抑制C3H10T1/2细胞的诱导分化。四甲氧基芪处理细胞后，关键核转录因子PPARγ的mRNA和蛋白表达呈剂量依赖性降低，其靶基因CD36和FABP4的mRNA表达也降低。
四甲氧基芪可通过抑制PPARγ抑制C3H10T1/2细胞的成脂分化
